【目的】 总结miR-152已有研究结果,并预测其可能参与的生物学过程,为本研究小组深入研究miR-152在非酒精性脂肪肝病(nonalcoholic fatty liver disease,NAFLD)形成和相关的调控机制奠定基础。 【方法】 PubMed检索miR-152相关文献,生物信息学预测miR-152的靶基因,并对其进行功能富集分析和信号通路富集分析。 【结果】 miR-152已在多个类型肿瘤、先兆子痫等疾病中有报道,但尚无参与脂肪肝的研究,分析结果显示miR-152靶基因功能集中于器官发育、细胞分化、代谢等多种分子功能和生物学过程;信号通路主要富集于多种肿瘤和细胞骨架蛋白、脂肪细胞因子、胰岛素信号及wnt信号通路中。 【结论】 miR-152在非酒精性脂肪肝病中尚未见报道,其预测的靶基因集合富集于多个生物学过程,为研究miR-152在非酒精性脂肪肝病中的作用提供了研究思路。
Abstract
【Objective】 To provide both theoretical and experimental basis for further study of NAFLD via reviewing the previous studies of miR-152 and bioinformatically predicting target genes of miR-152. 【Methods】 All literature of miR-152 was searched in PubMed.The predicted target genes of miRNA-152 were analysed by function enrichment (gene ontology) and signal transduction pathway enrichment (KEGG). 【Results】 miR-152 had been reported in studies of some cancers,preeclampsia and other diseases.The gene ontology analysis showed the predicted target genes of miR-152 were enriched in sensory organ developmentm,cell differentiation,metablism and other biological processes,and the pathway analysis showed the target genes mainly involved in cancers,actin cytoskeleton,adipocytokine,insulin signal and Wnt signaling pathway. 【Conclusions】 There's few study of miR-152 in NAFLD.The predicted target genes of miR-152 are enriched in multiple biological processes,which provid some clues for NAFLD.
关键词
miR-152 /
miRNA /
生物信息学分析 /
非酒精性脂肪肝病
Key words
miR-152 /
miRNA /
bioinformatics /
nonalcoholic fatty liver disease
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基金
十一五国家科技支撑计划(2009BAI80B01);浙江省医学重点学科(创新学科) (11-CX24);江苏省自然科学基金(BK2011107);南京市科技发展计划(201104013)
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