【目的】 探讨高压氧对宫内缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)新生大鼠脑组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malonyldiade-hyde,MDA)的影响及病理影响。 【方法】 按Bjelke法制作HIBD新生大鼠动物模型,随机分为HIBD组、HIBD+HBO组、对照鼠+HBO组和对照组4组,每组30只。HBO组大鼠于术后24 h开始给予2ATA的高压氧治疗,1 h/d,持续14 d。于出生后12 h、第15天各组大鼠随机取10只,分别处死取脑,制作脑组织匀浆,检测脑组织中SOD、MDA水平,并于第15天作HE和尼氏染色观察大鼠脑组织病理学变化。 【结果】 经HBO治疗后,HIBD+HBO组大鼠脑组织SOD升高[(90.43±10.83) U/mgprot]、MDA降低[(16.76±3.80) nmol/mgprot]、海马存活神经元数目增多[(122±10.52)个/0.2 mm2],与HIBD组[(74.18±8.52)U/mgprot、(28.05±4.28)nmol/mgprot、(92±6.51)个/0.2 mm2]比较差异均有统计学意义(P<0.05)。 【结论】 高压氧可通过拮抗氧自由基、减轻脑水肿和减少神经细胞死亡,发挥对HIBD新生鼠脑组织的保护作用。
Abstract
【Objective】 To explore the effects of hyperbaric oxygen (HBO) on the changes of superoxide dismutase(SOD),malonyldialdehyde (MDA) and pathology in brain tissue of infant rats with hypoxic-ischemic brain damage (HIBD). 【Methods】 The rat model of HIBD was made by the method of Bjelke and divided randomly into two groups (n=30)-HIBD group and HBO-treated HIBD group.Another 60 rats underwent sham-operation and were also divided randomly into HBO-treated the control group and the control group.After 24h of the operation,the rats of the HBO-treated groups received HBO (2ATA,1h/d) for 14 days.The rats were killed at postnatal 12h and 15d to detect the level of SOD and MDA in rat brain.HE staining and Nissl stain were employed to observe the pathological change and the number of neurons in hippocampus of rats when rats were 15 days old. 【Results】 In HIBD+HBO group,the level of SOD[ (90.43±10.83) U/mgprot] was higher than that in HIBD group [(74.18±8.52) U/mgprot]; but the level of MDA[ (16.76±3.80) nmol / mgprot ]was lower than that in HIBD group[(28.05±4.28)nmol/ mgprot].Surviving neurons of the HIBD+HBO rats[(122±10.52) cells /0.2 mm2] were more than the HIBD rats[(92± 6.51) cells/0.2 mm2] by Nissl's staining. 【Conclusion】 HBO might be useful to protect brain of neonatal rat against hypoxic-ischemic damage by resisting free redical,reducing brain edema and reducing neuron death.
关键词
大鼠 /
缺氧缺血性脑损伤 /
高压氧 /
氧自由基 /
神经细胞死亡
Key words
rats /
hypoxic-ischemic brain damage /
hyperbaric oxygen /
oxygen free radicals /
neuron death
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参考文献
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基金
福建省教育厅科研基金资助项目(JA01067)
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