【目的】 初步分析中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin,NGAL)水平对铁代谢指标血红蛋白(hemoglobin,HB)、铁蛋白(serum ferritin,SF)、血清转铁蛋白受体(serum transferrin receptor,sTfR)、转铁蛋白/铁蛋白指数(transferritin receptor-ferritin index,TFR-F)以及机体铁含量(body iron content,BIC)的关系。 【方法】 随机抽取473名3~6岁(不足7岁)学龄前儿童纳入本研究,利用氰化高铁血红蛋白法测定HB、ELISA法测定血清NGAL、SF及sTfR,并根据相关公式计算TRFI和BIC指数。 【结果】 血清NGAL与sTfR、TFR-F和BIC均呈显著相关关系(偏相关系数r=0.23、0.24、-0.15,P<0.01或<0.05)。贫血儿童血清NGAL水平显著低于非贫血儿童[(152.8±33.4)vs (220.7±25.4)pg/mL](P<0.05);以指标中位数为界,低sTfR组儿童血清NGAL水平明显低于高sTfR组[(181.05±41.1)vs(257.6±47.8)pg/mL](P<0.05),低BIC组儿童血清NGAL水平明显高于高BIC组[(269.1±49.4)vs(181.3±30.8)pg/mL](P<0.05)。低NGAL组儿童血清sTfR水平及TFR-F指数显著低于高NGAL组(P<0.05),而HB、SF以及BIC组间差异无统计学意义(P>0.05)。 【结论】 NGAL可能主要通过阻止红系统增殖参与调节儿童铁代谢稳态,而与机体铁储备过程无关。
Abstract
【Objective】 To explore the correlation of neutrophil gelatinase-associated lipocalin (NGAL) and iron metabolic index,including hemoglobin (HB),serum ferritin (SF),serum transferrin receptor (sTfR),transferritin receptor-ferritin index (TFR-F) and body iron content (BIC). 【Methods】 About 473 preschool children with 2~7 years old were randomly selected from eight kindergartens,including 223 boys and 250 girls with (47.8±14.5) months.HB was measured by HiCN method,NGAL,SF and sTfR by ELISA.TFR-F index and BIC were calculated according to the relevant index formula. 【Result】 There were significant correlation between NGAL and sTfR,TFR-F and BIC (the partial correlation coefficients were 0.23,0.24 and -0.15,P<0.01 or <0.05),respectively.The value of serum NGAL in anemic children was markedly higher than that of non-anemic children [(152.8±33.4) vs (220.7±25.4)pg/mL](P<0.05).Children with low sTfR level (cut by sTfR media value) had lower serum NGAL level than those with high sTfR level[(181.05±41.1) vs (257.6±47.8)pg/mL](P<0.05);however,children with low BIC level (cut by BIC media value) had higher serum NGAL level than those with high BIC level [(269.1±49.4) vs (181.3±30.8)pg/mL](P<0.05).The values of sTfR and TFR-F in children with low NGAL level (cut by NGAL media level) were significantly lower than those of children with high NGAL level (P<0.05),but not for HB,SF and BIC (P>0.05). 【Conclusion】 The impact of NGAL on iron metabolism may be mainly through the inhibition on erythroid proliferation,but no relationship with iron reserves process.
关键词
中性粒细胞明胶酶相关脂质运载蛋白 /
铁代谢 /
学龄前儿童 /
贫血
Key words
neutrophil gelatinase-associated lipocalin /
iron metabolism /
preschool children /
anemia
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参考文献
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基金
瑞士SIGHT AND LIFE国际维生素A研究基金(20090221)
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