【目的】 观察NYGGF4(PID1)基因过表达对脂肪细胞线粒体代谢的影响。 【方法】 以3T3-L1前体脂肪细胞为研究对象,体外培养稳定转染NYGGF4(PID1)基因(NYGGF4-pcDNA3.1 (+)/myc-His B)的3T3-L1前体脂肪细胞,以转染空载体[pcDNA3.1(+)/myc-His B]的3T3-L1前体脂肪细胞为对照,经1-甲基-3异丁基黄嘌呤(MIX)加地塞米松、胰岛素方案诱导分化为成熟脂肪细胞,采用Real-time PCR检测线粒体代谢酶指标:己糖激酶(Hexokinase,HKI)、乙酰辅酶A羧化酶(Acetyl-CoA,ACC)、柠檬酸合成酶( citrate synthase,CS)、肉毒碱棕榈酰转移酶1(Carnitine palmitoyl-transferase 1,CPT1)、细胞色素C(Cytochrome c,Cyc-c)基因表达水平。 【结果】 NYGGF4(PID1)过表达显著降低3T3-L1脂肪细胞己糖激酶、乙酰辅酶A羧化酶、柠檬酸合成酶、肉毒碱棕榈酰转移酶1、细胞色素C基因表达水平,差异有统计学意义。 【结论】 NYGGF4(PID1)基因过表达,下调3T3-L1脂肪细胞中定位于线粒体的代谢关键酶,提示NYGGF4(PID1)基因过表达能影响3T3-L1脂肪细胞的线粒体代谢。
Abstract
【Objective】 To explore the effect of NYGGF4 (PID1) gene over-expression on mitochondrial metabolism in adipocytes. 【Methods】 3T3-L1 preadipocytes transfected with either a NYGGF4 expression vector or an empty expression vector were induced to differentiate into adipocytes.The expression levers of metabolic enzymes in mitochondria-Hexokinase (HKI),Acetyl-CoA (ACC),citrate synthase (CS),Carnitine palmitoyl-transferase 1 (CPT1),Cytochrome C (Cyc-C) were detected by Real-time PCR. 【Result】 NYGGF4 over-expression was significantly lower level of metabolic enzymes in mitochondria-HKI,ACC,CS,CPT1,Cyc-c in adipocytes than control (NC) cells. 【Conclusion】 In adipocytes with NYGGF4 gene over-expression,mitochondria metabolic enzymes mRNA is down- regulated.It could implicate that mitochondrial metablism is influenced in adipocytes with NYGGF4 gene over-expression.
关键词
NYGGF4基因 /
线粒体 /
线粒体代谢 /
3T3-L1脂肪细胞
Key words
NYGGF4 gene /
mitochondria /
mitochondrial metabolism /
3T3-L1 adipocytes
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基金
国家自然科学基金(30973231,30571978);江苏省医学创新团队(LJ201108);江苏省六大人才高峰(卫生-39)
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