【目的】 探讨高压氧干预对丙戊酸钠(VPA)孤独症模型鼠学习记忆能力的影响,为高压氧治疗孤独症提供理论依据。 【方法】 通过Wistar大鼠怀孕第12.5天腹腔注射VPA的方法建立孤独症动物模型,采用Y型电迷宫测试评价高压氧干预对模型鼠学习记忆能力的影响。 【结果】 高压氧治疗孤独症模型组治疗后Y型电迷宫测试的尝试次数少于治疗前尝试次数(31.54±0.88 vs 30.69±0.63,t=5.500,P=0.001),再现次数多于治疗前再现次数(2.85±0.69 vs 3.38±0.65,t=2.214,P=0.047);常压高氧治疗孤独症模型组治疗后的尝试次数少于治疗前尝试次数(31.54±0.97 vs 31.15±0.99,t=2.739,P=0.018),再现次数多于治疗前再现次数(2.69±0.48 vs 3.00±0.58,t=2.309,P=0.040)。 【结论】 VPA孤独症动物模型建立成功,高压氧干预治疗可提高孤独症模型鼠的学习记忆能力。
Abstract
【Objective】 To explore the effect of hyperbaric oxygen on learning and memory functions in VPA autism rat model. 【Method】 Animal model of autism was obtained in offspring of the Wistar rats that received a single intraperitoneal injection of sodium valproate at the 12.5 day after pregnancy,the learning and memory of the autism model rats were evaluated by the Y electricity maze test before and after the hyperbaric oxygen intervention. 【Results】 The trying times of the hyperbaric oxygen group were fewer after treatment (31.54±0.88 vs 30.69±0.63,t=5.500,P=0.001) and the memory times were increased(2.85±0.69 vs 3.38±0.65,t=2.214,P=0.047);The trying times of the normobaric hyperoxia group were fewer after treatment(31.54±0.97 vs 31.15±0.99,t=2.739,P=0.018) and the memory times were increased(2.69±0.48 vs 3.00±0.58,t=2.309,P=0.040). 【Conclusion】 The VPA autism rats model is established successfully,the learning and memory abilities are improved after the hyperbaric oxygen intervention.
关键词
丙戊酸钠 /
高压氧 /
孤独症 /
学习 /
记忆
Key words
valproic acid /
hyperbaric oxygen /
autism /
learning /
memory
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基金
青岛大学医学院附属医院2010年院内重点培育学科专项建设基金项目(610)
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