新生儿感染性疾病血浆IL-6、TNF-α、NO追平变化及其意义的研究

魏朝霞

中国儿童保健杂志 ›› 2011, Vol. 19 ›› Issue (9) : 847-849.

临床研究与分析

新生儿感染性疾病血浆IL-6、TNF-α、NO追平变化及其意义的研究

魏朝霞

作者信息 +

Study on interleukin-6, tumor necrosis factor-α and nitic oxide in newborn with infection

WEI Zhao-xia

Author information +

文章历史 +

摘要

【目的】分析比较新生儿感染性疾病(败血症与一般感染) 血浆中部分细胞因子的变化特征。【方法】采用双抗体夹心酶联免疫吸附实验法(ELISA) 测定白细胞介素6 (interleukin, IL-6) 、肿瘤坏死因子α(tumer necrosis factor, TNF-α) 的水平,使用硝酸还原酶法测定NO水平。【结果】败血症患儿血浆IL-6、TNF-α、NO水平显著高于健康对照组(P<0.01),一般感染患儿血浆IL-6水平与健康对照组相比,差异无统计学意义( P>0.05)。新生儿缺血缺氧性脑病(hypoxic-ischemic encephalopathy,HIE)合并感染组TNF-α较单纯感染组差异无统计学意义(P>0.05),IL-6水平降低(P<0.01),而NO水平显著增高(P<0.01)。【结论】新生儿血浆中高水平的IL-6、TNF-α、NO提示机体存在感染, TNF-α可做为早期诊断的灵敏指标,并与感染轻重程度有关。新生儿窒息缺氧可影响IL-6和NO的水平。

Abstract

【Objective】 To study the characters of some cytokines levels in newborn with sepsis and infection. 【Method】 Cytokines were measured by sanwich enzyme-linked immunosorbent assay and nitric acid deoxidize method. 【Results】 The plasma IL-6, IL-8 and TNF-α levels in newborn with sepsis were significantly higher than the health control group (P<0.01). The plasma IL-6 levels in common infection had no difference with the health control group(P>0.05). There were no difference of TNFα between infection with hypoxic-ischemic encephalopathy(HIE) and pure infection(P>0.05), the levels of IL-6 were lower(P<0.01) and NO were higher (P<0.01)in the firster. 【Conclusions】 The high levels of IL-6, TNF-α and NO in newborn plasma indicate infection. TNF-α might be sensitive markers of diagnosis and degree of neonatal infection. The levels of IL-6, NO care be affected by asphyxia of newborn.

导出引用

魏朝霞. 新生儿感染性疾病血浆IL-6、TNF-α、NO追平变化及其意义的研究[J]. 中国儿童保健杂志. 2011, 19(9): 847-849

WEI Zhao-xia. Study on interleukin-6, tumor necrosis factor-α and nitic oxide in newborn with infection[J]. Chinese Journal of Child Health Care. 2011, 19(9): 847-849

中图分类号： R722

参考文献

[1] 中华医学会儿科学分会新生儿学组,中华医学会中华儿科杂志编辑委员会. 新生儿败血症诊疗方案[J]. 中华儿科杂志,2003,41(9):897-899.
[2] 何维.医学免疫学[M].北京:人民卫生出版社,2005:103-113.
[3] 冯小三,黄绍敏. 新生儿感染性疾病IL-26、IL-28和TNF-α的研究[J]. 中国优生与遗传杂志,2004,12 (1):93-95.
[4] Kurt AN,Aygun AD,Godekmerdan A,et al. Serum IL-1 beta, IL-6, IL-8, and TNF-alpha levels in early diagnosis and management of neonatal sepsis[J]. Mediators Inflamm,2007,2007:31397-31401.
[5] 华亚军,刘静,凌历,等. TNF-α和IL-2在新生儿全身炎症反应综合征中的临床价值[J].上海医药,2009,30(7):323-324.
[6] 吴素娟,邵肖梅,杨毅,等.新生儿细菌感染时血清TNF-α和IL-10水平变化[J].临床儿科杂志,2003,21(2):76-78.
[7] Schleifer KW,Mansfield JM.Suppressor macrophages in Arican trypanosomiasis T cell prdiferative responses by nitric oxide and prostaglandins[J].J Immunol,1993,151(10):492-503.
[8] 陈珊,张洪,陈达光,等.新生儿败血症血清NO^-₂ /NO^-₃和cGMP的变化[J].华中医学杂志,1998,22(4):171-172.
[9] Santana C,Guindeo MC,Gonzalez G,et al.Cord blood levels of cy2 tokines as predictors of early neonatal sepsis[J].Acta Paediatr,2001,90 (10):1176-1181.