【目的】 探讨单纯性肥胖儿童载脂蛋白E(apolipoprotein E, ApoE)基因多态性的频率分布及对其血脂谱水平的影响。 【方法】 采用基因测序方法对100例单纯性肥胖儿童及100例正常对照组儿童进行ApoE基因多态性测定,另外抽取空腹静脉血作甘油三酯(triglyceride, TG)、胆固醇(total cholesterol, TC)、高密度脂蛋白(high-density lipoprotein cholesterol, HDL)、低密度脂蛋白(low-density lipoprotein cholesterol, LDL)、载脂蛋白A(apolipoprotein A, ApoA)、载脂蛋白B(apolipoprotein B, ApoB)检测,观察不同基因型和等位基因的分布情况,并与血脂的相关性进行分析。 【结果】 基因型E3/3频率分布最高,在70%之上,而含E3的杂合子(E2/3和E3/4)居中,二者之和超过20%,E2/2和E4/4表型的频率最低;与对照组比较,实验组肥胖儿童E2型等位基因有较高水平的TG(P＜0.05),E3型有较高的TG、TC、LDL水平和较低HDL、ApoA水平(P＜0.01),E4型有较高的TG、AopB水平和较低的HDL、ApoA水平(P＜0.05);肥胖组儿童中,与E3相比较,E4具有较高的TG、ApoB值和较低的HDL值,差异有统计学意义(P＜0.05),而E2具有较低的TC、LDL和ApoB水平,但是差异无统计学意义;携带不同等位基因的肥胖儿童的血脂异常率为E4＞E3＞E2(P＜0.01)。 【结论】 ApoE3/3是分布频率最高的基因型,ApoE4等位基因是血脂异常的风险基因之一,与肥胖儿童的血脂异常存在显著相关性。
Abstract
【Objective】 To explore the frequency distribution of apolipoproteinE(ApoE) gene polymorphism of obesity children and its effection on lipid levels in plasma. 【Methods】 Using DNA sequencing method to detect the apolipoproteinE gene polymorphism of 100 obesity children and 100 normal children of control group. Moreover, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), apolipoprotein A(apoA) and apolipoprotein B(apoB) were detected with the 12 h fasting blood. Observing the distribution of different genotypes and allele, and analysis of its correlation with lipid. 【Results】 E3/3 genotype frequence(＞70%) of ApoE was quite higher than E3 heterozygous (E2/3 and E3/4＞20%) and E2/2and E4/4; compared to the control group, E2 allele of the experimental group had higher level of TG(P＜0.05), E3 allele had higher level of TG, TC, LDL and lower level of HDL, ApoA(P＜0.01), while E4 allele had higher level of TG, AopB and lower level of HDL, ApoA(P＜0.05), the differences had statistical significance; in experimental group, compared to E3 allele, the child who carry E2 allele had lower level of TC, LDL and ApoB(P＞0.05), E4 allele has higher level of TG, ApoB and lower HDL(P＜0.05); the obesity children who carry different allele had different dyslipidemia rate, it was E4＞E3＞E2(P＜0.01). 【Conclusion】 The apoE3/3 is the most common genotype, and ApoE4 are correlated with dyslipidemia of obesity children and is one of the risk genes of dyslipidemia.
关键词
载脂蛋白E /
肥胖儿童 /
基因多态性 /
血脂
Key words
apolipoproteinE /
obesity children /
polymorphism /
lipid
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