【目的】 建立致敏佝偻病动物模型并检测补充维生素D(vitamin D,VD)前后25-(OH)D3、IgE、IFN-γ及IL-4变化,为儿科临床佝偻病及过敏性疾病防治提供实验依据。 【方法】 选用SD大鼠,建立致敏佝偻病模型,随机分为补D 30万U组(7只)、补D 60万U组(7只)、对照组(6只),实验组采用一次性肌注补D方法,饲养20 d天后采用ELLISA方法及流式细胞仪检测大鼠过敏及免疫指标。 【结果】 补D组较对照组比较,30万U维生素D可使致敏佝偻病SD大鼠外周血过敏指标IgE降低(P＜0.01),而60万U维生素D则使IgE上升(P＜0.01);30万U组IFN-γ的UR值上升(P＜0.05),但仍较正常鼠低,IL-4的UR值有所下降(P＜0.05);60万U组IFN-γ的UR值不但没有上升,反而下降更加明显(P＜0.05), IL-4的UR值则上升明显(P＜0.05)。 【结论】 一次性肌注30万U维生素D可以改善过敏机体的免疫状态,而当剂量达60万U时则加重过敏机体Th1/Th2细胞失衡,进一步加重机体的过敏反应。
Abstract
【Objective】 To establish an animal model of rickets that sensitized for measuring their concentration include 25-(OH)D3、IgE、IFN-γ、IL-4 in venous blood after supply vitamin D, as a reference in prophylaxis and treatment of rickets and systemic anaphylaxis disease in pediatry. 【Methods】 The SD rats model of rickets that sensitized were divided into 3 groups in experiment include 3×105 U doses of vitamin D group (7 rats),6×105 U doses of vitamin D group (7 rats) 、control group(6 rats), all the way to a muscle column for experimental groups ,the method include of Elisa and flow cytometry used to test allergy and immunity target of rats after raising 20 days. 【Results】 The experimental groups compared with the control group, 3×105 U doses of vitamin D decreased the allergy target -IgE of the SD rats(P＜0.01), but 6×105 U doses of vitamin D made IgE increased(P＜0.01),the UR value of IFN-γ of small dose group increased(P＜0.05),but still under normal rats, the UR value of IL-4 decreased; the UR value of IFN-γ of 6×105 U doses group was not only ascend, but also descend evidently(P＜0.05),but the UR value of IL-4 increased(P＜0.05). 【Conclusion】 3×105 U doses of vitamin D could improve this unbalance , but 6×105 U doses can intensify Th1/Th2 unbalance of allergy body, further increase the physical allergy .
关键词
维生素D /
IFN-γ /
IL-4 /
Ig-E /
Th1/Th2 /
致敏佝偻病SD大鼠
Key words
the rickets rat after sensitization /
vitamins D /
IFN-γ /
IL-4 /
Th1/Th2
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基金
黑龙江省攻关课题基金(GA07C303109)
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