目的 探讨参芍胶囊对宫内感染早产仔鼠血清炎性因子及其学习记忆能力的影响,为降低早产儿学习记忆能力损伤提供新思路。方法 60只孕鼠随机分为两组(实验组及对照组),孕1日开始,实验组每日使用浓度为25mg/mL参芍胶囊溶液、对照组使用等量生理盐水进行预灌胃,剂量根据人与大鼠体表面积换算。孕17日,实验组、对照组均宫内注射100μL脂多糖(LPS,25μg/μL)溶液。孕18日两组孕鼠中分别随机选取15只孕鼠断颈处死,ELISA法检测仔鼠血清中IL-4、IL-10的改变。每组剩余的15只孕鼠等待自然分娩,观察妊娠结局后随机选取10只仔鼠待其1月龄使用Morris水迷宫实验评价仔鼠学习记忆能力。结果 使用参芍胶囊干预后,实验组与对照组相比,早产率(χ2=14.122)与死产率(χ2=7.266)降低,活产仔鼠数增加(t=5.386),差异有统计学意义(P<0.05)。实验组与对照组间仔鼠血清IL-4、IL-10水平差异有统计学意义(t=5.652、6.273,P<0.05)。定位航行实验中,实验组仔鼠较对照组更多地采取了有效策略(χ2=2.568,P<0.05),平均潜伏期亦较对照组仔鼠明显缩短(F=64.320,P<0.05);空间探索实验中,实验组较对照组停留时间明显缩短,差异有统计学意义(t=3.038,P<0.05)。结论 参芍胶囊可减轻早产仔鼠体内炎性反应,改善仔鼠学习记忆能力。
Abstract
Objective To investigate the effects of Shenshao Capsule on serum inflammatory factors and learning-memory abilities in preterm offspring rats induced by intrauterine infection, in order to provide new insights for mitigating learning and memory impairments in preterm infants. Methods Sixty pregnant rats were randomly divided into two groups (experimental group and control group).From gestational day 1, the experimental group received a daily gavage of Shenshao Capsule solution (25mg/mL), while the control group received an equivalent volume of normal saline.Dosages were adjusted based on human-to-rat body surface area conversion.On gestational day 17, both groups were intrauterinely injected with 100μL of lipopolysaccharide (LPS, 25μg/μL) solution.On gestational day 18, 15 pregnant rats from each group were randomly selected and euthanized by cervical dislocation.ELISA was used to measure changes in serum IL-4 and IL-10 levels in the offspring.The remaining 15 pregnant rats in each group were allowed to deliver naturally.After observing pregnancy outcomes, 10 offspring from each group were randomly selected for Morris water maze testing at one month of age to assess learning and memory abilities. Results Compared with the control group, the experimental group exhibited significantly lower preterm birth rate (χ2=14.122),and decreased stillbirth rate (χ2=7.266),higher live birth count (t=5.386) (P<0.05).Serum IL-4 and IL-10 levels in the experimental offspring were significantly different from those in the control group (t=5.652, 6.273, P<0.05).In the navigation test, the experimental offspring adopted more effective strategies (χ2=2.568, P<0.05) and demonstrated significantly shorter mean escape latencies (F=64.32, P<0.05).In the spatial probe test, the experimental group spent significantly less time in the target quadrant (t=3.038, P<0.05). Conclusions Shenshao Capsule alleviates inflammatory responses in preterm offspring rats and improves their learning and memory capacities.
关键词
早产 /
子代 /
学习记忆 /
参芍胶囊
Key words
premature birth /
offspring /
learning-memory /
Shenshao Capsule
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基金
陕西省重点研发计划项目(2023-YBSF-231);步长-西安交通大学第一附属医院联合基金项目(BC2017-08);西安交通大学第一附属医院院基金项目(2021ZXY-13);优生科学协会-本真公益基金项目(BZ2020-02)。
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