目的 分析miRNA-155在哮喘预测指数(API)阳性和阴性反复喘息儿童中的表达水平,探讨微小RNA-155(miRNA-155)与喘息及嗜酸性粒细胞计数的关系。方法 选取2019年1月—2020年10月在玉环市人民医院住院治疗的反复喘息儿童40例为喘息组,其中API阳性儿童27例,API阴性儿童13例;同时选择健康体检儿童40例为对照组。检测喘息组和对照组儿童外周血miRNA-155、嗜酸性粒细胞、IFN-γ和IL-4水平。结果 喘息组miRNA-155水平、IFN-γ/IL-4值显著低于对照组 (t＝2.307,3.839,P＜0.05),喘息组嗜酸性粒细胞数明显高于对照组(t =5.278,P＜0.05);喘息组中API阳性儿童miRNA-155水平、IFN-γ/IL-4值显著低于API阴性及对照组儿童(H =13.580,F=9.731,P＜0.05),喘息组中API阳性儿童嗜酸性粒细胞明显高于API阴性及对照组儿童(F=22.303,P＜0.05);喘息组中API阳性儿童miRNA-155水平与喘息次数呈负相关(r＝-0.566,P＜0.05),喘息组miRNA-155水平与嗜酸性粒细胞计数呈负相关(r＝-0.630,P＜0.05)。结论 miRNA-155水平下降可能是喘息发生的危险因素,且miRNA-155在喘息发生发展中的作用可能与嗜酸性粒细胞有关。
Abstract
Objective To analyze the expression level of microRNA-155(miRNA-155) in asthmatic predictive index (API) positive and negative children with recurrent wheezing, and to explore the relationship between miRNA-155 and wheezing as well as eosinophil count. Methods A total of 40 children with recurrent wheezing in Yuhuan People′s Hospital from January 2019 to October 2020 were enrolled in the wheezing group, including 27 API positive children and 13 API negative children. Meanwhile, 40 healthy children were selected into the control group. The levels of miRNA-155, eosinophils, IFN-γ and IL-4 in peripheral blood of wheezing group and control group were tested. Results The levels of miRNA-155 and IFN-γ/IL-4 in wheezing group were significantly lower than those in control group(t=2.307, 3.839, P<0.05), while the number of eosinophils in wheezing group was significantly higher than that in the control group(t =5.278, P＜0.05). The miRNA-155 level and the IFN-γ/IL-4 value of API positive children in the wheezing group were significantly lower than those of API negative and control children (H=13.580, F=9.731,P<0.05). In the wheezing group, the number of eosinophils of API positivechildren was significantly higher than that of API negative and control children(F=22.303, P＜0.05). In the wheezing group, the level of miRNA-155 in API positive children was negatively correlated with the number of wheezing(r＝-0.566, P<0.05).The level of miRNA-155 was negatively related to eosinophil count in the wheezing group(r＝-0.630, P<0.05). Conclusion The decrease of miRNA-155 level may be a risk factor for wheezing, and the role of miRNA-155 in wheezing development may be related to eosinophils.
关键词
微小RNA-155 /
喘息 /
哮喘预测指数 /
儿童 /
哮喘
Key words
microRNA-155 /
wheezing /
asthma predictive index /
children /
asthma
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参考文献
[1] 王慧敏,刘传合.年幼儿童反复喘息发作的远期预后及影响因素[J].中华实用儿科临床杂志,2019,34(12):957-959.
[2] 沈仁,李孟荣,张林桃,等.哮喘预测指数与婴幼儿首次毛细支气管炎及以后反复喘息的相关性观察[J].中国儿童保健杂志,2017,25(3):293-296.
[3] 代香兰,兀威,冯梅.MicroRNA-155在支气管哮喘中的研究进展[J].国际呼吸杂志,2018,38(2): 118-121.
[4] Malmhäll C, Johansson K, Winkler C, et al.Altered miR-155 expression in allergic asthmatic airways[J].Scand J Immunol, 2017, 85(4):300-307.
[5] Naro na B, Langwiński W, Szczepankiewicz A.Non-Coding RNAs in Pediatric Airway Diseases[J].Genes (Basel), 2017, 8(12):348.
[6] 中华医学会儿科学分会呼吸学组,《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南[J].中华儿科杂志,2008,46(10):745-753.
[7] 蒋沈华,虞坚尔,薛征,等.哮喘发病机制中起调控作用的miRNA 研究进展[J].现代中西医结合杂志,2020,29(17): 1913-1917.
[8] 庄鹏晖,刘亮亮,郑国玺,等.支气管哮喘患者血清肿瘤坏死因子-α、白细胞介素-8和嗜酸性粒细胞阳离子蛋白水平变化及意义[J].陕西医学杂志,2020,49(12):1597-1599,1603.
[9] Backman K,Nuolivirta K, Ollikainen H,et al.Low eosinophils during bronchiolitis in infancy are associated with lowerriskof adulthood asthma[J].Pediatr Allergy Immunol 2015,26(7):668-673.
[10] Panganiban RP,Wang Y,Howrylak J,et al. Circulating microRNAs as biomarkers in patients with allergic rhinitis and asthma[J].J Allergy Clin Immun,2016,137 (5) : 1423-1432.
基金
浙江省医药卫生科技计划项目(2019KY795)
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