目的 研究白细胞介素-33/肿瘤发生抑制蛋白2(IL-33/ST2)在肺炎支原体肺炎(MPP)患儿外周血中的表达及临床意义。方法 选取2017年6月-2018年10月开封市儿童医院收治MPP患儿100例为研究对象(MPP组),另取同期健康体检者儿童100例为对照组,采集两组儿童外周静脉血,分离外周血单个核细胞(PBMC)及血浆,采用酶联免疫吸附试验(ELISA)检测IL-33、IL-4、IFN-γ表达水平,免疫印迹法(WB)检测ST2蛋白表达水平,COSMED PFT4型仪器检测两组儿童肺功能指标,采用Pearson法分析MPP患儿外周血IL-33、ST2蛋白水平与肺功能相关性。结果 与对照组比较,MPP儿童外周血IL-33、ST2蛋白水平、IL-4水平显著增加(t=9.749、 33.783、 43.890, P＜0.05),INF-γ水平、PEF%、FEV1%、FEV1/FVC水平均显著降低(t=12.014、 19.860、 20.258、 21.006, P＜0.05)。Pearson法分析结果显示,MPP儿童外周血中IL-33、ST2水平与IL-4水平呈正相关(r=0.537、 0.511),与INF-γ水平、PEF%、FEV1%、FEV1/FVC水平均呈负相关(r=-0.660~-0.431,P＜0.05)。结论 IL-33、ST2在MPP患儿外周血中高表达,可能通过介导Th2优势免疫应答参与MPP气道炎症反应。
Abstract
Objective To investigate the expression of interleukin-33/tumor suppressor protein 2 (IL-33/ST2) in peripheral blood of children with mycoplasma pneumonia (MPP) and its clinical significance. Methods From June 2017 to October 2018, 100 children with MPP in Kaifeng Children's Hospital were selected as the study subjects (MPP group), and another 100 healthy children in the same period were selected as the control group.Peripheral venous blood was collected from two groups of children, peripheral blood mononuclear cells (PBMC) and plasma were isolated.The expression of IL-33 was detected by enzyme-linked immunosorbent assay (ELISA), the expression of ST2 protein was detected by immunoblotting (WB), COSMED PFT4 instrument was used to detect pulmonary function in two groups of children.Pearson method was used to analyze the correlation between IL-33 and ST2 protein levels in peripheral blood of MPP children and pulmonary function. Results Compared with the control group, the levels of IL-33 and IL-4, ST2 protein in peripheral blood of MPP children increased significantly (t=9.749, 33.783, 43.890, P<0.05), while the levels of INF-γ, PEF%, FEV1% and FEV1/FVC decreased significantly (t=12.014, 19.860, 20.258, 21.006, P<0.05).Pearson analysis showed that IL-33 and ST2 protein levels in peripheral blood of MPP children were positively correlated with IL-4 level(r=0.537, 0.511), and negatively correlated with INF-γ level, PEF%, FEV1%, FEV1/FVC level (r=-0.660—-0.431, P <0.05). Conclusions IL-33 and ST2 are highly expressed in peripheral blood of MPP children.It may be involved in airway inflammation of MPP by mediating Th2 dominant immune response.
关键词
肺炎支原体肺炎 /
白细胞介素-33 /
肿瘤发生抑制蛋白2 /
肺功能
Key words
mycoplasma pneumoniae pneumonia /
interleukin-33 /
tumor inhibitor protein 2 /
lung function
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基金
2018 年河南省科技研发专项(182102310177)
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