目的 探讨瘦素处理对慢性束缚应激(CRS)造成的青春期小鼠焦虑样行为的影响,为青春期情绪障碍的治疗提供研究资料。方法 选取50只21~30 d龄小鼠,连续10 d 低强度(2 h)/高强度(4 h)束缚应激,高架十字迷宫(EPM)和矿场实验(OFT)观察大鼠焦虑样行为,酶联免疫法检测血清瘦素水平。结果 高强度束缚(HCRS)组小鼠血清瘦素含量为(4.2±1.3) pg/ml,低于对照组小鼠[(10.4±2.9)pg/ml](P<0.05)。EPM实验中,HCRS小鼠在开放臂停留时间和进入开放臂次数分别为(68.5±18.9)s和1.7±1.1,少于正常组小鼠[(101.3±19.8)s,3.8±1.8,P< 0.01],而瘦素处理后,这两个指标分别升高至(93.8±16.4)s和3.3±1.6,接近正常水平。OFT实验中,HCRS小鼠在中央区停留时间为(63.4±15.9)s,低于对照组[(142.5±20.2)s,P<0.01],应用瘦素后该时间延长至(130.5±26.5)s。而低强度束缚小鼠在这两个实验中的各指标均未出现明显改变。结论 HCRS可以造成青春期小鼠的焦虑障碍,慢性瘦素应用提高了血清瘦素含量,改善了焦虑症状。
Abstract
Objective To explore the effect of leptin treatment on anxiety-like behavior caused by chronic restraint stress in adolescent mice,in order to provide data for the treatment of adolescent emotional disorders. Methods Totally 50 mice aged 21-30 days were subjected to low-intensity (2 h) / high-intensity (4 h) restraint stress for 10 consecutive days.Anxiety-like behavior was observed by elevated plus maze (EPM) and open field test (OFT).Serum leptin level was measured by enzyme-linked immunosorbent assay. Results Serum leptin level in the high-intensity restraint stress (HCRS) group[(4.2±1.3) pg/ml]was lower than that in the control group[(10.4±2.9)pg/ml,P<0.05].In the EPM experiment,the open arm times and the open arm entries in the HCRS group were (68.5±18.9)s and 1.7±1.1,respectively,significantly less than those in the normal group[(101.3±19.8) s,3.8±1.8,P<0.01).After the treatment of leptin,the above two indexes increased to (93.8±16.4)s and 3.3±1.6,respectively,which were close to the normal level.In the OFT experiment,the time spent in central area of HCRS mice[(63.4±15.9)s]was lower than that of the control group[(142.5±20.2) s,P<0.01],and this index was prolonged to (130.5±26.5)s after the application of leptin.However,there was no significant change in the indexes of the adolescent mice experienced low-intensity restraint stress in the two experiments. Conclusion HCRS can cause the anxiety disorder of adolescentmice,and using chronic leptin increases serum leptin levels and improve anxiety symptom.
关键词
焦虑 /
瘦素 /
高架十字迷宫 /
慢性束缚应激
Key words
anxiety /
leptin /
elevated plus maze /
chronic restraint stress
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基金
陕西省自然科学基金项目(2020JM-054);西安市科技计划项目[2017118SF/YX012(6)];陕西省软科学研究计划项目(2020KRM060)
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