目的 分析孤独症谱系障碍(ASD)儿童肠道菌群多样性,为减轻胃肠道症状提供科学依据。方法 选取2018年1-6月在广西壮族自治区妇幼保健院确诊为ASD的儿童20例和健康儿童20例作为研究对象。采集两组儿童粪便提取总DNA,扩增16S rDNA中V4/V5区进行高通量测序,分析ASD儿童肠道菌群多样性。结果 两组在样品文库的覆盖率(Z=242.500,P=0.256)、丰度指标Chao1(Z=250.000,P=0.181)、 ACE(Z=234.000,P=0.365)、Shannon(Z=259.000,P=0.114)和Simpson(Z=146.000,P=0.145)指数上差异均无统计学意义(P>0.05),多样性无差异。门水平上,两组儿童在8种菌群上差异无统计学意义(P>0.05),属水平上,两组儿童在20个属上差异具有统计学意义(P＜0.05)。结论 ASD儿童和健康儿童在肠道菌群属水平上具有差异;临床上可根据不同菌属特点对ASD儿童肠道菌群进行精准调整,保持肠道微生态平衡,减轻ASD儿童胃肠道症状。
Abstract
Objective To analyze the structure and diversity of intestinal flora in children with autism spectrum disorder (ASD). Methods The number of 20 children with ASD diagnosed in Maternal Child Health Hospital of Guangxi Autonomous Region and the number of 20 healthy children from January to June 2018 were selected as the subjects in the study.Total DNA was extracted from feces of two groups of children,and V4/V5 region of 16S rDNA was amplified for high throughput sequencing.The intestinal flora diversity of ASD children was analyzed by sequencing results. Results There were no significant differences in the coverage of sample libraries (Z=242.500,P=0.256),abundance index Chao1 (Z=250.000,P=0.181),ACE (Z=234.000,P=0.365),Shannon (Z=259.000,P=0.114) and Simpson (Z=146.000,P=0.145) indices between the two groups (P>0.05).At the gate level,there was no significant difference between the two groups in 8 species of bacteria (P>0.05),but at the generic level,there was significant difference between the two groups in 20 genera (P<0.05). Conclusions There are differences in intestinal flora between ASD children and healthy children.In clinic,the intestinal flora of ASD children can be accurately adjusted according to the characteristics of different bacteria genus to maintain the intestinal microecological balance and alleviate the gastrointestinal symptoms of ASD children.
关键词
肠道菌群 /
孤独症谱系障碍 /
多样性 /
儿童 /
高通量测序
Key words
intestinal flora /
autism spectrum disorder /
diversity /
children /
high throughput sequencing
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基金
中国疾病预防控制中心妇幼保健中心2017年儿童早期发展适宜技术项目(2017FYE006)
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