目的 研究CTLA-4基因多态性与儿童支气管哮喘易感性及外周血IFN-γ、IL-4和IgE的关系,为儿童支气管哮喘的诊断及治疗提供理论及临床依据。方法 2017年4月-2019年2月选择郑州大学第三附属医院儿童支气管哮喘患者78例为病例组,健康体检的正常儿童67例作为对照组,检测两组CTLA-4 基因的rs231725单核苷酸多态性,同时检测病例组外周血IFN-γ、IL-4、IgE水平。结果 病例组CTLA-4 rs231725 AA基因型比例高于对照组(66.7% vs. 19.4%),CTLA-4 rs231725 GG基因型比例低于对照组(19.3% vs. 73.2%)(P<0.05);病例组CTLA-4 rs231725 A等位基因频率比例高于对照组(73.7% vs. 23.1%)(P<0.05),CTLA-4 rs231725 G等位基因频率比例低于对照组(26.3% vs. 76.9%)(P<0.05);CTLA-4 rs231725 AG基因型IFN-γ、IL-4、IgE水平均高于GG基因型,差异有统计学意义(P<0.05);CTLA-4 rs231725各基因型及等位基因频率与有无支气管哮喘伴泌尿系统损害、支气管哮喘伴消化道反应无明显相关关系(P>0.05);CTLA-4 rs231725 AA基因型(OR=2.625,95%CI=1.273~5.138)及A等位基因频率(OR=1.987,95%CI=1.121~3.397)与支气管哮喘伴肾炎相关关系明显(P<0.05)。结论 CTLA-4 rs231725 AA基因型和A等位基因均与儿童支气管哮喘易感性增加相关;高水平的IFN-γ、IL-4、IgE可能是CTLA-4 rs231725 AA基因支气管哮喘易感性增强的原因。
Abstract
Objective To explore the relationship between CTLA-4 gene polymorphism and susceptibility to bronchial asthma in children and peripheral blood IFN-γ, IL-4 and IgE, and provide theoretical and clinical basis for the diagnosis and treatment of bronchial asthma in children. Methods From April 2017 to February 2019,78 children with bronchial asthma in the Third Affiliated Hospital of Zhengzhou University were in the case group, and 67 healthy children in the physical examination were used as the control group.The rs231725 single nucleotide polymorphism of CTLA-4 gene was detected in both groups, and the levels of IFN-γ, IL-4 and IgE in peripheral blood of the case group were also detected. Results The proportion of CTLA-4 rs231725 AA genotype was higher in the case group than in the control group (66.7% vs 19.4%), and the proportion of CTLA-4 rs231725 GG genotype was lower than that in the control group (19.3% vs 73.2%) (P<0.05); CTLA-4 rs231725 A allele frequency ratio was higher than in the control group (73.7% vs 23.1%) (P＜0.05), and the CTLA-4 rs231725 G allele frequency ratio was lower than in the control group (26.3% vs 76.9%) (P <0.05); CTLA-4 rs231725 AG genotype IFN-γ, IL-4, IgE levels were higher than GG genotype, and the differences were statistically significant (P<0.05); At CTLA-4 rs231725 genotypes and allele frequencies, there were no significant correlation between bronchial asthma and urinary system damage, bronchial asthma with digestive tract reaction (P>0.05).CTLA-4 rs231725 AA genotype(OR=2.625,95%CI=1.273-5.138) and A allele(OR=1.987,95%CI=1.121-3.397) frequencie were associated with bronchial asthma with nephritis(P<0.05). Conclusion CTLA-4 rs231725 AA genotype and A allele are associated with increased susceptibility to bronchial asthma in children, which may be caused by the high levels of IFN-γ, IL-4, and IgE.
关键词
CTLA-4基因多态性 /
支气管哮喘 /
易感性 /
IFN-γ /
IL-4 /
IgE
Key words
CTLA-4 gene polymorphism /
bronchial asthma /
susceptibility /
IFN-γ /
IL-4 /
IgE
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基金
河南省医学科技攻关计划项目(201703219)
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