目的 分析不同类型过敏原对哮喘患儿T淋巴细胞亚群的影响,以期为哮喘防治提供参考。方法 对147例支气管哮喘急性发作患儿进行过敏原筛查,应用流式细胞术检测其CD3、CD4、CD8、CD4/CD8水平。结果 本研究中哮喘患儿平均发病年龄为1岁11个月,男性患儿(72.1%)较女性患儿(27.9%)多,儿童过敏性哮喘以环境过敏阳性为主。环境过敏原阳性组外周血CD4水平与过敏原阴性患儿之间的差异有统计学意义(χ2=10.317,P=0.006)。不同类型过敏原哮喘患儿外周血CD3、CD8、CD4/CD8之间的差异无统计学意义(P>0.05)。结论 环境过敏原致敏更易影响哮喘儿童免疫功能的改变,造成CD4 T淋巴细胞平衡失调,在哮喘患儿免疫功能判断和临床防治中应重点关注。
Abstract
Objective To analyze the effect of different types of allergens on T lymphocyte subsets of children with asthma.Methods Totally 147 cases of asthma children were collected to detect the allergens and flow cytometry was conducted to test immune indices levels, such as CD3, CD4, CD8, CD4/CD8.Results Children developed asthma at the age of one year and eleven months averagely. The proportion of male cases (72.1%) was higher than that of female cases (27.9%). Environmental allergy was found predominant in childhood with allergic asthma. The CD4 level of children with environmental allergy was significantly different from that without environmental allergy (χ2=10.317, P=0.006), while significant difference was not found in term of CD3, CD4, CD8, CD4/CD8 levels (P>0.05).Conclusion Environmental allergy is prone to influence children′s immune function, resulting in CD4 T lymphocyte out of balance. So it is supposed to pay more attention to environmental allergy in judging the immune function of asthma children and clinical prevention and treatment.
关键词
哮喘 /
过敏原 /
T淋巴细胞亚群 /
儿童
Key words
asthma /
allergen /
T Lymphocyte subsets /
children
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 王卫平.儿科学[M].8版.北京:人民卫生出版社,2013:271.
[2] Asher I,Pearce N.Global burden of asthma among children[J].Int J Tuberc Lung Dis,2014,18(11):1269-1278.
[3] 刘传合, 洪建国, 尚云晓,等. 中国16城市儿童哮喘患病率20年对比研究[J]. 中国实用儿科杂志,2015,30(8):596-600.
[4] 中华医学会儿科分会呼吸学组.2008年修订儿童支气管哮喘防治常规[J].中华儿科杂志,2008,46(10):745-753.
[5] 赵凯, 徐红梅, 叶侃,等. 苏州城区支气管哮喘儿童特异性过敏原检测的意义[J]. 中华实用儿科临床杂志, 2007, 22(4):279-280.
[6] Mclellan K, Shields M, Power U, et al. Primary airway epithelial cell culture and asthma in children-lessons learnt and yet to come[J]. Pediatr Pulmonol,2015,50(12):1393-1405.
[7] 李敏, 张琼, 时维娟,等. 成都地区0～14岁城乡儿童哮喘流行病学调查对比分析[J].中国当代儿科杂志,2013,15(8):609-613.
[8] 李莉, 刘建梅, 陈强,等. 南昌市城区0-14岁儿童哮喘流行病学调查结果分析[J]. 江西医药,2011,46(11):1051-1052.
[9] 杭金国, 姚泽忠, 管敏昌,等. 台州地区支气管哮喘患儿过敏原特异性IgE检测分析及临床意义[J]. 检验医学,2011,26(9):627-629.
[10] Milligan KL, Matsui E, Sharma H. Asthma in urban children:epidemiology, environmental risk factors, and the public health domain[J]. Curr Allergy Asthma Rep, 2016, 16(4):33-36.
[11] 郭惠环, 林少玲. 孟鲁司特对儿童支气管哮喘患者T淋巴细胞亚群检测的影响[J]. 黑龙江医学, 2015, 39(8):935-936.
[12] Shurin MR, Yanamala N, Kisin ER, et al. Graphene oxide attenuates Th2-type immune responses, but augments airway remodeling and hyperresponsiveness in a murine model of asthma.[J]. Acs Nano, 2014, 8(6):5585-5599.
[13] 陈阵, 王恩洁, 管敏昌. 哮喘患儿外周血淋巴细胞亚群和CD4+T细胞亚群的变化情况[J]. 中华全科医学, 2017, 15(5):811-813.
[14] 董昭兴, 康庆鑫, 雷雯,等. IL-17对肺成纤维细胞的增殖、转化和胶原合成作用[J]. 南方医科大学学报, 2012, 32(1):75-79.
[15] Al-Daghri NM, Alokail MS, Draz HM, et al. Th1/Th2 cytokine pattern in Arab children with severe asthma[J]. Int J Clin Exp Med, 2014, 7(8):2286-2291.
PDF(486 KB)
