目的 探讨炎性细胞因子白介素-6(IL-6)、IL-6R、IL-1β、IL-1RN基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的严重程度的相关性,为重症HFMD的早期发现和患病风险的评估提供依据。方法 以西安市儿童医院和西安交通大学第二附属医院感染科收住的180例EV71 HFMD患儿为研究对象,其中重症组108例,普通组72例。采用SNPscanTM多重SNP技术检测上述样本IL-6 rs1800796、rs2069837,IL-6R rs2228145、rs4845617,IL-1β rs16944、rs1143627,IL-1RN rs315952、rs9005位点的基因分型。结果 180例EV71感染患儿中,IL-6 rs1800796 G等位基因的重症化风险升高(OR=1.312,95%CI:1.041~1.653),IL6-R rs2228145 C等位基因重症化风险升高(OR=1.330,95%CI:1.064~1.663),而IL-1β rs1143627 G等位基因是保护因素(OR=0.790,95%CI:0.635~0.981)。IL-6 rs2069837、IL-6R rs4845617,IL-1β rs16944、IL-1RN rs315952、rs9005的基因多态性与疾病严重程度无明显相关性。结论 IL-6 rs1800796、IL-6R rs2228145、IL-1β rs1143627基因多态性与EV71 HFMD患儿严重程度相关联。
Abstract
Objective To investigate the association of inflammatory cytokines including interleukin-6 (IL-6), IL-6R, IL-1β and IL-1RN gene polymorphism with severity of hand foot and mouth disease (HFMD) induced by enterovirus 71 (EV71), in order to provide evidence for early recognition and assessment of severe HFMD.Methods A total of 180 EV71 HFMD cases including 108 severe cases and 72 mild cases from the Second Affiliated Hospital of Xi′an Jiaotong University and Xi′an Children′s Hospital were randomly enrolled in this study. All participants were genotyped for the most common IL-6, IL-6R, IL-1β and IL-1RN single-nucleotide polymorphisms (SNPs) using the SNPscan multiple SNP typing method. The gene type of rs1800796, rs2069837 of IL-6, rs2228145, rs4845617 of IL-6R,rs16944, rs1143627 of IL-1β and rs315952, rs9005 of IL-1R among 180 cases were detected by SNPscan kit.Results The severity risk was significantly increased in IL-6 rs1800796 allele G(OR=1.312, 95% CI:1.041~1.653)and IL6-R rs2228145 allele C (OR=1.330, 95% CI:1.064～1.663), while it was reduced in IL-1β rs1143627 allele G (OR=0.790,95% CI:0.635~0.981). No association of gene polymorphism with severity of EV71 HFMD was observed in IL-6 rs2069837, IL-6R rs4845617, IL-1β rs16944, IL-1RN rs315952 and IL-1RN rs9005.Conclusion The gene polymorphism of IL-6 rs1800796, IL-6R rs2228145 and IL-1β rs1143627 were associated with severity of EV71 HFMD.
关键词
肠道病毒71型 /
白介素-6 /
白介素-1β /
基因多态性 /
重症化
Key words
enterovirus 71 /
interleukin-6 /
interleukin-1β /
polymorphism /
severity
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基金
国家自然科学基金(81701632);陕西省社会发展攻关项目(2017SF-154)
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