目的 研究不同糖耐量肥胖儿童血清硫化氢(H2S)及25-羟基维生素D3[25-(OH)D3]水平的变化及意义,为临床治疗提供参考依据。方法 2013年1月-2016年1月收集204例口服葡萄糖耐量试验(OGTT)结果异常肥胖儿童的资料,根据OGTT结果将其分为空腹血糖受损(IFG)组、糖耐量受损(IGT)组、IFG合并IGT组以及糖尿病(DM)组。选择同期46例OGTT正常的肥胖儿童为单纯肥胖组,分别通过分光光度计法检测H2S水平,酶联免疫法检测25-(OH)D3水平。结果 IFG组、IGT组、IFG合并IGT组以及DM组血清H2S及25-(OH)D3水平均低于单纯肥胖组(P＜0.05);IFG合并IGT组血清H2S及25-(OH)D3均低于IFG组、IGT组(P＜0.05);DM组血清H2S及25-(OH)D3水平均低于IFG组、IGT组及IFG合并IGT组(P＜0.05);IFG组血清H2S低于IGT组(P＜0.05)。DM组血清H2S及25-(OH)D3水平均低于单纯肥胖组(P＜0.05);IFG合并IGT组血清H2S及25-(OH)D3水平均低于单纯肥胖组(P＜0.05)。相关性分析中,单纯肥胖组、IFG组、IGT组、IFG合并IGT组以及DM组血清H2S及25-(OH)D3与0 h PG、2 h PG、HBALC水平均呈显著性负相关(all P＜0.05);单纯肥胖组、IFG组、IGT组、IFG合并IGT组以及DM组血清H2S与25-(OH)D3水平与呈显著正相关( P＜0.05)。结论 血清H2S及25-(OH)D3水平与肥胖儿童糖耐量情况密切相关,其水平的明显下降可能是肥胖儿童发展为2型糖尿病的危险因素。
Abstract
Objective To study the significance of changes of serum hydrogen sulfide and 25-(OH)D3 in obese children with different glucose tolerance. Methods According to the oral glucose tolerance test(OGTT),204 obese children were divided into impaired fasting glycaemia(IFG) group,impaired glucose tolerance (IGT) group,IFG combined with IGT group and diabetes mellitus(DM) group from Jan 2013 to Jan 2016. Totally 46 obese children with normal OGTT were as obese group. The levels of serum H2S and 25-(OH)D3 were tested by spectrophotometer and euzymelinked immunosorbent assay(ELISA) respectively. Results The levels of H2S and 25-(OH)D3 were lowest in obese group (P＜0.05);The levels of H2S and 25-(OH)D3 were lower in IFG combined with IGT group than IFG group and IGT group (P＜0.05);The levels of H2S and 25-(OH)D3 were lower in DM group than those of IFG group,IGT group and IFG combined with IGT group(P＜0.05); The levels of H2S and 25-(OH)D3 were lower in IFG group than IGT group(P＜0.05). The levels of H2S and 25-(OH)D3 were lower in DM group than obese group (P＜0.05); The levels of H2S and 25-(OH)D3 were lower in IFG combined with IGT group than obese group and IGT group (P＜0.05).The levels of serum H2S and 25-(OH)D3 was negatively correlated with 0 h PG,2 h PG and HBALC (P<0.05). The level of serum H2S was positively correlated with 25-(OH)D3(P<0.05). Conclusion Serum levels of H2S and 25-(OH)D3 are closely associated with glucose tolerance in obese children and it may develop into a risk factor when the levels decrease obviously.
关键词
2型糖尿病 /
硫化氢 /
25-羟基维生素D3
Key words
diabetes mellitus type 2 /
H2S /
25-(OH)D3
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参考文献
[1] Tura A,Gobl C,Moro E,et al.Insulin resistance and beta-cell dysfunction in people with prediabetes according to criteria based on glycemia and glycosylated hemoglobin[J].Endocr J,2017,64(1):117-122.
[2] 王剑清,刘艳明.维生素D调控儿童肥胖、胰岛素抵抗及其机制的研究进展[J].中华实用儿科临床杂志,2015,30(20): 1598-1600.
[3] Moreira-Lucas TS,Duncan AM,Rabasa-Lhoret R,et al.Effect of vitamin D supplementation on oral glucose tolerance in individuals with low vitamin D status and increased risk for developing type 2 diabetes (EVIDENCE):A double-blind,randomized,placebo-controlled clinical trial[J].Diabetes Obes Metab,2016,19(1):133-141.
[4] Wilinski B,Wilinski J,Somogyi E,et al.Vitamin D3 (cholecalciferol) boosts hydrogen sulfide tissue concentrations in heart and other mouse organs[J].Folia Biol(Krakow),2012,60(3-4):243-247.
[5] 李辉,季成叶,宗心南,等.中国0~18岁儿童、青少年体块指数的生长曲线[J].中华儿科杂志,2009,47(7):493-498.
[6] 刘戈力,姜丽红.儿童和青少年2型糖尿病诊治进展[J].中华实用儿科临床杂志,2015,30(20): 1525-1528.
[7] 张远达,顾芳,谢会卿,等.轻度胃肠炎伴婴幼儿良性惊厥患儿血清硫化氢水平变化的研究[J].中国当代儿科杂志,2014,16(11):1096-1099.
[8] Xue Y,Gao M,Gao Y.Childhood type 2 diabetes: Risks and complications[J].Exp Ther Med,2016,12(4):2367-2370.
[9] Bernabé-Ortiz A,Carrillo-Larco RM,Gilman RH,et al.Geographical variation in the progression of type 2 diabetes in Peru: The cronicas cohort study[J].Diabetes Res Clin Pract,2016,121:135-145.
[10] Sciacqua A,Perticone M,Grillo N,et al.Vitamin D and 1-hour post-load plasma glucose in hypertensive patients[J].Cardiovasc Diabetol,2014,20(13):48.
[11] Al-Shoumer KA,Al-Essa TM.Is there a relationship between vitamin D with insulin resistance and diabetes mellitus?[J].World J Diabetes,2015,6(8):1057-1064.
[12] Peterson CA,Tosh AK,Belenchia AM.Vitamin D insufficiency and insulin resistance in obese adolescents[J].Ther Adv Endocrinol Metab,2014,5(6):166-189.
[13] 朱翔,周剑,朱文静,等.不同糖耐量人群血清维生素D水平的变化[J].中国临床保健杂志,2016,19 (3):243-245.
[14] Kimura H.Hydrogen sulfide and polysulfides as signaling molecules[J].Proc Jpn Acad Ser B Phys Biol Sci,2015,91(4):131-159.
[15] Sindhu S,Koshy M,Al-Roub AA,et al.Differential association of plasma monocyte chemoattractant protein-1 with systemic inflammatory and airway remodeling biomarkers in type-2 diabetic patients with and without asthma[J].J Diabetes Metab Disord,2016,15:40.
[16] Hajmrle C,Smith N,Spigelman AF,et al.Interleukin-1 signaling contributes to acute islet compensation[J].JCI Insight,2016,1(4):e86055.
[17] Zhang H,Zhuang XD,Meng FH,et al.Cells from high glucose & methylglyoxal-induced injury through restoration of CBS/H2S expression[J].Neurochem Int,2016,92:49-57.
[18] 张远达,冀超玉,王静,等.川崎病患儿硫化氢与白细胞介素1β、白细胞介素6水平的变化及其相关性[J].中华实用儿科临床杂志,2015,30(21):1676-1677.
[19] Sommer A,Fabri M.Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-Producing T Cells[J].PLoS One,2015,10(6):e0130395.
[20] Jain SK,Manna P,Micinski D,et al.In African American type 2 diabetic patients,is vitamin D deficiency associated with lower blood levels of hydrogen sulfide and cyclic adenosine monophosphate,and elevated oxidative stress?[J].Antioxid Redox Signal,2013,18(10):1154-1158.
基金
河北省科技计划项目(172777129); 保定市科技支撑计划项目(17ZF062)
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