目的 分析布拉氏酵母菌联合早期干预对高胆红素血症仔鼠脑组织NF-kB表达和学习记忆功能的影响。 方法 将足月7日龄SD大鼠88只随机分为对照组(NS组)和试验组(T1、T2、T3组),每组22只。试验组于7日龄和10日龄分别腹腔注射胆红素50 μg/g,对照组注射等量生理盐水。于末次注射12 h后,各组随机选取6只,测定血清胆红素和脑组织胆红素含量。剩余仔鼠,T1组单纯给予布拉氏酵母菌,T2组给予布拉氏酵母菌和早期干预,T3组不干预,至28日龄。HE染色和免疫组化观察NF-kB表达情况,Morris水迷宫评测仔鼠逃避潜伏时间和穿过目标象限次数。 结果 10日龄NS组血清胆红素和脑组织胆红素浓度显著低于试验组(P<0.01),试验组间差异无统计学意义(P>0.05);28日龄NS组显著低于T1、T2、T3组(P<0.05),T2组显著低于T1、T3组(P<0.05)。HE染色:NS组神经元结构完整,T1、T2、T3组均观察到神经元数量减少,不同程度的胆红素沉积。免疫组化:NS组NF-kB阳性细胞数明显少于T1、T2、T3组(P<0.01),T2组明显少于T1组及T3组 (P<0.05) 。NS组逃避潜伏时间和穿过目标象限次数显著优于T1、T2、T3组(P<0.05),T2组显著优于T1组及T3组(P<0.05)。 结论 布拉氏酵母菌联合早期干预可有效地减少高胆红素血症仔鼠脑组织NF-kB表达,改善学习记忆功能。
Abstract
Objective To discuss the effect of saccharomyces boulardii and early intervention on NF-kB expression in brain tissue, as well as learning and memory in neonatal rats with hyperbilirubinemia. Methods A total of 88 SD rats aged 7 days were randomly divided into the control group (NS group) and experiment group (T1,T2 and T3 group), with 22 rats in each group. The experiment group was intraperitoneally injected with 50 μg/g bilirubin at 7 days and 10 days respectively, and the control group was injected intraperitoneally with equal volume of normal saline. After the last injection of 12 h, 6 neonatal rats were randomly selected in each group, and the bilirubin contents in the brain and serum were measured. Meantime, group T1 was treated with saccharomyces boulardii, and group T2 was treated with saccharomyces boulardii and early intervention, group T3 was untreated till 28 days old. HE was used to observe the pathological changes of brain tissue, the NF-kB positive cells were observed by immunohistochemical staining, and morris water was used to evaluate the latency and the times crossing target quadrants. Results The bilirubin contents in the brain and serum of NS group with 10-day-old rats were significantly lower than those in the experiment group (P<0.01), but there was no significant difference among the experiment groups (P>0.05). The bilirubin contents in the brain and serum of NS group with 28-day-old rats were significantly lower than those in group T1, T2 and T3, and the bilirubin contents in the brain and serum in group T1 were significantly lower than those in group T1 and T3 (P<0.05). HE results showed that the neurons of NS group had complete structure, but the number of neurons in group T1,T2 and T3 were decreased and with bilirubin deposits. The number of NF-kB positive cells in NS group were significantly less than those in group T1,T2 and T3 (P<0.01), and were also less in group T2 than that in group T1 and T3 (P<0.05). The latency and the times crossing target quadrants in NS group were significantly better than those in group T1、T2 and T3 (P<0.05), and were also better in group T2 than that in group T1 and T3 (P<0.05). Conclusion Saccharomyces boulardii and early intervention can effectively reduce expression of NF-kB in brain tissue and improve learning and memory in neonatal rats with hyperbilirubinemia.
关键词
高胆红素血症 /
布拉氏酵母菌 /
早期干预 /
核因子KappaB /
学习记忆 /
大鼠
Key words
hyperbilirubinemia /
saccharomyces boulardii /
early intervention /
NF-kB /
learning and memory /
rats
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