目的 观察不同强度的运动训练对青春期大鼠学习记忆能力的影响以及和血清瘦素水平的关系,为研究青春期学习记忆能力的提高提供依据。方法 选择30只45~50日龄大鼠,随机分为低强度运动组、中等强度运动组和对照组,每组10只,连续一周低强度或中等强度跑台运动训练,Morris水迷宫观察大鼠学习记忆,酶联免疫法检测血清瘦素(Lep)水平。结果 中等强度运动训练组大鼠Morris水迷宫实验四个象限的平均逃避潜伏期分别为(44.8±5.0)s,(49.3±5.2)s,(39.3±4.7)s和(37.7±2.8) s,均短于正常组大鼠(P<0.01),中等强度运动训练组大鼠穿越站台次数为5.2±1.8,高于正常组大鼠(2.6±0.4),差异均具有统计学意义(P<0.01)。中等强度运动组大鼠的血清Lep水平[(15.1±4.5) pg/ml]高于正常组大鼠[(12.8±3.7) pg/ml](t=2.47,P<0.05)。结论 中等强度的运动训练能够提高青春期大鼠的学习记忆能力,这可能与血清Lep含量的增加有关。
Abstract
Objective To observe the effect of different intensities of exercise training on learning and memory of adolescent rats,as well as the relationship between training and serum leptin levels. Methods Totally 30 rats aged 45-50 days were selected and randomly divided into three groups,including low intensity exercise,moderate intensity exercise and control group,with 10 rats in each group. Morris water maze was used to observe the learning and memory of rats. The serum leptin level was tested by enzyme-linked immunosorbent assay (ELISA). Results The average escape latency of four morris water maze tests in the moderate intensity exercise was (44.8±5.0) s,(49.3±5.2) s,(39.3±4.7) s and (37.7±2.8) s,respectively,which were all shorter than those of control group(P＜0.01). The number of crossing platform and the level of serum leptin in the moderate intensity exercise training group were significantly higher than those in normal group (15.1±4.5) pg/ml vs. (12.8±3.7) pg/ml,5.2±1.8 vs. 2.6±0.4,P<0.05). Conclusion Moderate intensity exercise training can improve learning and memory ability of adolescent rats,which may be related to the increase of serum leptin content.
关键词
学习记忆 /
运动训练 /
瘦素 /
青春期大鼠
Key words
learning and memory /
exercise training /
leptin /
adolescent rats
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参考文献
[1] van Praag H,Christie BR,Sejnowski TJ,et al. Running enhances neurogenesis,learning,and long-term potentiation in mice[J].PNAS,1999,96 (23):13427-13431.
[2] 娄淑杰,刘瑾彦,杨若愚,等. 跑台运动促进幼龄大鼠学习能力[J].生理学报,2006,58 (4):365-369.
[3] Murray DK,Sacheli MA,Eng JJ,et al. The effects of exercise on cognition in Parkinson's disease:a systematic review[J].Transl Neurodegener,2014,3(1):5.
[4] Obeid M,Frank J,Medina M,et al.Neuroprotective effects of leptin follow ing kainic acid-induced status epilepticus [J].Epilepsy Behav,2010,19(3):278-283.
[5] Li XM,Yan HJ,Guo YS,et al. The role of leptin in central nervous system diseases[J].Neuroreport,2016,27(5):350-355.
[6] Jin L,Li YP,Feng Q,et al. Cognitive deficits and Alzheimer-like neuropathological impairments during adolescence in a rat model of type 2 diabetes mellitus[J].Neural Regen Res,2018,13(11):1995-2004.
[7] 付燕,张业廷,罗笑,等. 有氧运动对Aβ1-42 诱导阿尔茨海默病大鼠学习记忆能力及海马炎症状态的影响[J].中国运动医学杂志,2018,37(8):676-684.
[8] 安楠. 运动对成长期骨骼肌神经源性诱导因子Agrin 及其受体MuSK表达的影响[J].中国运动医学杂志,2017,36(10):896-900.
[9] 崔嘉琴,柏友萍,林潺. 不同运动强度结合共轭亚油酸对青春期肥胖大鼠静息能量代谢的影响[J].四川体育科学,2017,36(1):33-39.
[10] Otgon-Uul Z,Suyama S,Onodera H,et al. Optogenetic activation of leptin- and glucose-regulated GABAergic neurons in dorsomedial hypothalamus promotes food intake via inhibitory synaptic transmission to paraventricular nucleus of hypothalamus[J].Mol Metab,2016,5(8):709-715.
[11] Yada T,Scott MM,Lachey JL,et al.Leptin targets in the mouse brain [J].J Comp Neurol,2009,514 (5):518-532.
[12] Guimond D,Diabira D,Porcher C,et al. Leptin potentiates GABAergic synaptic transmission in the developing rodent hippocampus[J].Front Cell Neurosci,2014,8(235):1-17.
[13] Moult P,Harvey J. Hormonal regulation of hippocampal dendritic morphology and synaptic plasticity [J].Cell Adh Migr,2008,2(4):269-275.
[14] Moult PR,Cross A,Santos SD,et al.Leptin regulates AMPA receptor trafficking via PTEN inhibition [J].J Neurosci,2010,30 (11):4088-4101.
基金
陕西省自然科学基金项目(2016JM8011);西安市科技计划项目(2017118SF/YX012(6))
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