目的 探讨小肠型脂肪酸结合蛋白(FABP2)基因G54A多态性与儿童单纯性肥胖发病的相关性,为减少儿童肥胖的发生提供科学依据。方法 2014年1月-2017年2月选取重庆市第三人民医院和重庆市第四人民医院收集的重庆市渝中区两路口地区80例单纯性肥胖患儿作为肥胖组,按照1∶1配对选取80例体重正常的健康儿童作为对照组,采用PCR技术检测两组的FABP2基因G54A多态性,检测两组的血生化相关指标并进行比较,采用Logistic多因素回归分析法探讨FABP2基因G54A基因多态性与儿童单纯性肥胖的关系。结果 肥胖组和对照组儿童血清总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖、脂肪酸水平差异无统计学意义(P>0.05),肥胖组甘油三酯(TG)、瘦素、皮质醇、内脏脂肪特异性丝氨酸蛋白酶抑制剂显著高于对照组(P<0.05),肥胖组脂联素(APN)显著低于对照组(P<0.05);肥胖组FABP2基因G54A位点等位基因A频率(43.13%)显著高于对照组(27.50%)(P<0.05);儿童血清TG(OR＝1.528,95%CI:1.221~4.007)、瘦素(leptin)(OR＝1.694,95%CI:1.381~4.214)、皮质醇水平升高(OR＝1.664,95%CI:1.360~4.815)、等位基因A增加(OR＝1.773,95%CI:1.468~5.598),APN的水平降低(OR＝0.728,95%CI:0.517~0.839)是儿童单纯性肥胖的独立危险因素(P<0.05)。结论 FABP2基因G54A多态性中等位基因A是儿童单纯性肥胖的独立危险因素。
Abstract
Objective To investigate the association between fatty acid binding protein 2(FABP2)G54A polymorphism and childhood simple obesity. Methods A total of 80 simple obesity children from Chongqing No.3 and No.4 People Hospitals of Chongqing City were enrolled in obesity group, and 80 normal healthy children were selected as the control group according to the 1∶1 pairings from January 2014 to February 2017. PCR technology was used to detect FABP2 gene G54A polymorphism in both groups.Blood biochemical indicators were detected and compared.And the relationship between FABP2 gene G54A gene polymorphism and childhood simple obesity was analyzed by Logistic regression analysis. Results There were no significant differences on serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG) and fatty acid (FFA) between obesity group and control group (P>0.05). The serum levels of triglyceride (TG), leptin, cortisol and visceral fat specific serine protease inhibitors in obesity group were significantly higher than those in control group (P<0.05),but the adiponectin (APN) level in obesity group was significantly lower than that in control group (P<0.05). The FABP2 gene G54A allele frequency of A (43.13%) in obesity group was significantly higher than that in control group (27.50%) (P<0.05); Serum TG(OR＝1.528,95%CI:1.221~4.007), leptin(OR＝1.694,95%CI:1.381~4.214), increased cortisol levels(OR＝1.664,95%CI:1.360~4.815) and allele A(OR＝1.773,95%CI:1.468~5.598), as well as the decreased level of APN(OR＝0.728,95%CI:0.517~0.839) were risk factors for simple obesity in children (P<0.05). Conclusion The G54A polymorphism of FABP2 gene A is an independent risk factor for simple obesity in children.
关键词
小肠型脂肪酸结合蛋白 /
多态性 /
儿童 /
单纯性肥胖
Key words
small intestinal fatty acid binding protein /
polymorphism /
children /
simple obesity
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 徐云鹏, 饶小娇, 郝敏,等. FABP2基因G54A多态性对单纯性肥胖儿童糖脂代谢的影响[J]. 卫生研究,2016,45(1):1-7.
[2] 徐海冬, 桑艳梅, 闫洁,等. 新发现的肥胖症相关基因单核苷酸多态性与中国儿童肥胖症的相关性[J]. 中国糖尿病杂志,2015,12(9):557-561.
[3] 林玉玲, 金文波, 黄文平,等. A2BP1基因内含子1单核苷酸多态性与肥胖的相关性分析[J]. 山东医药,2016,56(14):72-74.
[4] 王桂兰, 王冰洁, 陈德晖,等. 支气管哮喘患儿ORMDL3基因单核苷酸多态性对其发病的影响[J]. 中华实用儿科临床杂志,2015,30(4):282-285.
[5] 郭明飞, 高家荣, 姜辉,等. 2型糖尿病模型大鼠肝脂代谢相关基因的筛选及生物信息学分析(英文)[J]. 中国生物化学与分子生物学报,2017,17(7):726-735.
[6] 代东伶, 周少明, 柳国胜,等. 脂质代谢相关基因变异与肥胖儿童非酒精性脂肪肝易感性分析[J]. 临床儿科杂志, 2017, 35(4):241-246.
[7] Heida FH, Hulscher JB, Schurink M, et al. Intestinal fatty acid-binding protein levels in necrotizing enterocolitis correlate with extent of necrotic bowel: results from a multicenter study[J]. Pediatr Surg,2015,50(7):1115-1118.
[8] 张震, 富文俊, 邢宇锋,等. L-FABP/PPARα信号通路与非酒精性脂肪性肝炎关系的研究进展[J]. 山东医药,2016,56(8):98-100.
[9] Kitai T, Kim YH, Kiefer K, et al. Circulating intestinal fatty acid-binding protein (i-fabp) levels prortends higher risk of adverse clinical outcomes in advanced decompensated heart failure[J]. J Am Coll of Cardiol, 2017, 69(11):950.
[10] 荣光辉, 徐琪, 徐铁山,等. 脂肪型和瘦肉型北京鸭A-FABP基因组织表达特征[J]. 中国畜牧杂志, 2015, 51(1):5-9.
[11] Matsumoto S, Sekine K, Funaoka H, et al. Diagnostic value of intestinal fatty acid-binding protein for pneumatosis intestinalis[J]. Am J Surg,2016,212(5):961-968.
[12] 邢媛媛, 李大彪, 李红磊,等. 催乳素对奶牛乳腺上皮细胞乳脂和乳蛋白合成相关基因表达的影响[J]. 动物营养学报,2016,28(8):2439-2447.
[13] 赵生国, 唐红杰, 焦婷,等. 早胜牛A-FABP基因SNPs与胴体品质和肉质性状的关系[J]. 草业科学,2015,32(7):1150-1155.
[14] Matsumoto S, Sekine K, Funaoka H, et al. Early diagnosis of hollow viscus injury using intestinal fatty acid-binding protein in blunt trauma patients[J]. Medicine,2017,96(10):e6187.
PDF(455 KB)
