目的 探讨白细胞介素-8(IL-8)基因多态性与呼吸道合胞病毒诱发的急性支气管炎发生发展的相关性,为预测疾病发展提供科学依据。方法 2017年3－9月选取本院收治的80例呼吸道合胞病毒诱发的急性支气管炎患儿(病例组)以及30名健康儿童(对照组),根据疾病严重程度,将病例组分为轻症组(n=52)与中重症组(n=28)。采用酶联免疫吸附试验(ELISA)法检测三组受试者血清IL-8表达;采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术测定三组受试者IL-8基因-251、781多态性位点基因型,计算基因型频率与等位基因频率并进行比较。结果 对照组受试者血清IL-8表达[(4.35±0.84)] pg/ml低于其余两组,轻症组[(7.63±1.31)] pg/ml低于中重症组[(10.22±1.85)] pg/ml(P<0.05)。对照组受试者IL-8基因-251位点AA基因型频率(6.67%)低于其余两个病例组(23.08%、28.57%)(P<0.05)。对照组受试者IL-8基因-251位点A等位基因频率(33.33%)低于其余两个病例组(51.92%、55.38%)(P<0.05)。连锁分析结果显示,IL-8基因-251位点A等位基因与781位点C等位基因为紧密连锁,其共同组成的AC单体型与呼吸道合胞病毒诱发的急性支气管炎疾病易感性以及严重程度相关。结论 IL-8基因-251、781位点的AC单体型与呼吸道合胞病毒诱发的急性支气管炎发生发展相关。
Abstract
Objective To investigate the association between interleukin-8 gene polymorphism and occurrence and development of acute bronchitis induced by respiratory syncytial virus,in order to provide scientific reference for predicting the development of acute bronchitis. Methods Totally 80 children with acute bronchitis induced by respiratory syncytial virus(case group) and 30 healthy children (control group) who underwent physical examination were selected as the participants from March to September in 2017.According to the severity of the disease,children in case group were divided into mild group (n=52) and moderate to severe group (n=28).The expression of IL-8 in three groups was tested by enzyme linked immunosorbent assay (ELISA),and the genotype frequencies of-251 and 781 polymorphic loci of three groups of IL-8 genes were measured by PCR-RFLP. Results The serum expression of IL-8 in control group [(4.35±0.84)pg/ml] was lower than that in the other two groups.The expression of IL-8 in mild group was lower than that in moderate and severe group [(7.63±1.31)pg/ml vs.(10.22±1.85)pg/ml,P<0.05)].The AA genotype frequency of IL-8 gene-251 locus in control group (6.67%) was lower than that in the other two cases (23.08%,28.57%) (P<0.05).Likewise,the frequency of allele A of IL-8 gene-251 locus in control group (33.33%) was lower than that in the other two groups (51.92%,55.38%) (P<0.05).Linkage analysis indicated that the A allele of the IL-8 gene-251 loci was closely linked to the 781 locus C allele,and the common AC haplotype was associated with the susceptibility to acute bronchitis caused by respiratory syncytial virus and the severity of the disease. Conclusion The AC haplotype of-251 and 781 loci of IL-8 gene is associated with the occurrence and development of acute bronchitis induced by respiratory syncytial virus.
关键词
白细胞介素-8 /
单核苷酸多态性 /
呼吸道合胞病毒 /
急性支气管炎
Key words
interleukin-8 /
singlenucleotidepolymorphisms /
respiratorysyncytialvirus /
acutebronchitis
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