目的 评估铁缺乏对患儿淋巴细胞增殖功能及细胞因子分泌水平的影响,初步探讨缺铁性贫血(IDA)导致儿童免疫功能下降的原因。方法 2018年3月4日-2018年4月28日选取IDA患儿和健康体检患儿各20例,作为IDA组和正常对照组,MTT比色法检测两组儿童T、B淋巴细胞在10、20、50 μmol/L铁浓度培养液下淋巴细胞体外刺激指数(SI),ELISA法检测两组儿童在不同铁浓度培养液下 单个核细胞(PBMC) INF-γ、IL-2、IL-6、IL-10的分泌水平。结果 1)对照组20 μmol/L铁浓度培养液下B、T淋巴细胞SI值高于其他浓度组,且高于同浓度组IDA患儿,IDA组在给予生理浓度铁补充下SI值有所升高;2)IDA组各铁浓度下INF-γ、IL-2分泌水平均显著低于对照组,同组中20 μmol/L浓度组显著高于10 μmol/L浓度组;3)IDA组10、20 μmol/L浓度组IL-6、IL-10分泌水平均显著高于对照组。结论 IDA患儿T、B淋巴细胞增殖功能障碍以及细胞因子分泌水平的改变可能是导致患儿免疫功能失衡,免疫力下降的原因之一。
Abstract
Objective To evaluate the effect of iron deficiency on lymphocyte proliferation and cytokine secretion in children with iron deficiency anemia (IDA),and to explore the causes of the decline of immune function in children caused by IDA. Methods Totally 20 IDA children were selected in IDA group and 20 healthy children were selected as control group from March 4th to April 28th in 2018. The lymphocyte stimulating index under 10μmol/L,20 μmol/L and 50 μmol/L iron supplementation from IDA group and control group was examined by MTT. The production of INF-γ,IL-2,IL-6 and IL-10 by peripheral blood mononuclear cells (PBMC) under different iron supplementation was examined by ELISA. Results 1) In the control group,SI of B and T cells under iron concentration of 20 μmol/L was significantly higher than that in other iron concentration levels,and was also significantly higher than that under iron concentration of 20 μmol/L in IDA group. Moreover,the SI of IDA children was significantly increased after physiological concentration iron supplementation.2) PBMC from IDA patients showed significantly lower production of INF-γ and IL-2 at all iron concentration levels than PBMC from healthy controls,but INF-γ and IL-2 levels at the concentration of 20 μmol/L were significantly higher than those at the concentration of 10 μmol/L group.3) The secretory levels of IL-6 and IL-10 in IDA group were significantly higher than those in control group at the concentration of 10 μmol/L and 20 μmol/L. Conclusion The changes of T,B lymphocyte proliferation and cytokine secretion in IDA children may be one of the reasons for the imbalance of immune function and weakening immune system
关键词
缺铁性贫血 /
淋巴细胞增殖 /
细胞因子 /
儿童
Key words
iron deficiency anemia /
lymphocytes proliferation /
cytokine /
children
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