目的 分析儿童外周血白细胞形态参数的变化及其临床意义。方法 设定运用Sysmex XE-2100全自动血细胞分析仪(简称XE-2100分析仪)检验血常规的180例门诊表观健康查体儿童(年龄≤15岁)为研究对象,回顾性收集其白细胞形态参数数据。按照年龄0～岁、1～岁、2～岁、6～岁、12～15岁分组,分析研究各白细胞形态参数的变化。结果 研究观察各年龄组儿童中性粒细胞散射荧光度即颗粒度(NEUT-X)为1 355.0±41.5、1 348.3±22.5、1 348.4±51.3、1 339.3±51.6、1 335.8±46.2,各组之间差异无统计学意义(P=0.292);中性粒细胞核酸荧光强度即核的大小(NEUT-Y)为495.6±28.2、477.6±38.6、486.5±40.2、481.3±32.2、479.9±39.6,各组之间差异无统计学意义(P=0.412);淋巴细胞核酸荧光强度(LYMPH-Y)为713.6±46.3、708.3±60.3、711.0±61.2、712.8±72.3、711.6±75.3,各组间差异无统计学意义(P=0.115);淋巴细胞散射荧光强度(LYMPH-X)为896.3±36.2、854.6±19.3、858.4±17.6、860.1±15.7、851.3±18.2,各组间差异有统计学意义(P=0.008),其中0～岁组与其他各组两两比较差异有高度统计学意义(P＜0.001)。结论 小于1岁的健康儿童外周血淋巴细胞颗粒度较其他年龄健康儿童高,具有较高的自身免疫抵抗能力。
Abstract
Objective To analyze the morphological parameters of peripheral blood leucocytes in children,and to find the clinical significance.Methods A total of 180 healthy children (under 15 years old) were enrolled in this study,and parameters of white blood cells were analyzed by Sysmex XE-2100 automatic blood analyzer.The parameter data and changes of white blood cells were analyzed by age 0～,1～,2～,6～,12～15 years old.Results The fluorescence of neutrophilic granulocytes (NEUT-X) of all age groups were 1 355.0±41.5,1 348.3±22.5,1 348.4±51.3,1 339.3±51.6,1 335.8±46.2 respectively,and there were no statistical difference among groups (P=0.292).The fluorescence intensity of neutrophilic nucleic acid (NEUT-Y) were 495.6±28.2,477.6±38.6,486.5±40.2,481.3±32.2,479.9±39.6 respectively,and there were no statistical differences (P=0.412).The fluorescence intensity of lymphocyte nucleic acid (LYMPH-Y) were 713.6±46.3,708.3±60.3,711.0±61.2,712.8±72.3,711.6±75.3,and there were no significiant differences among groups (P=0.115).The fluorescence intensity of lymphocyte(LYMPH-X) were 896.3±36.2,854.6±19.3,858.4±17.6,860.1±15.7,851.3±18.2,and there were statistical differences among groups (P=0.008).Conclusion The granularity of peripheral blood lymphocyte in healthy children under 1 year old is higher than that of other ages,which indicated 0~1 years old children have better immunity.
关键词
白细胞形态参数 /
儿童外周血 /
淋巴细胞颗粒度 /
大颗粒淋巴细胞
Key words
leukocyte morphological parameters /
peripheral blood of children /
lymphocyte granularity /
macrogranular lymphocytes
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