目的 探讨过氧化物酶增殖体激活受体-γ2基因(PPARγ2)单核苷酸多态性位点rs1801282与壮族儿童单纯性肥胖的相关性。方法 利用SNaPshot技术分析收集到的285例儿童(151例广西柳州壮族单纯性肥胖儿童和134例健康对照儿童)PPARγ2基因rs1801282位点的基因型,检测并比较两组的身高、体重和脂肪代谢相关生化指标(血糖、总胆固醇、甘油三脂、高密度脂蛋白和低密度脂蛋白)。结果 正常对照组和肥胖组PPARγ2基因CC型及CG+GG型基因型频率分别为95.52%、4.48%和98.01%、1.99%,两组最小等位基因(G等位基因)频率分别为2.24%和0.99%,两组基因型与等位基因频率差异均无统计学意义(P>0.05);rs1801282位点各基因型间代谢指标(FBG、TC、TG、HDL-C和VDL-C)差异无统计学意义(P>0.05)。按性别分组分析,结果差异均无统计学意义(P>0.05)。结论 PPARγ-2基因rs1801282位点多态性与广西壮族儿童单纯性肥胖无关。
Abstract
Objective To explore the relationship of single nucleotide polymorphisms(SNP) rs1801282 between PPARγ2 gene and simple obesity in Zhuang nationality children. Methods A total of 285 children(151 cases of simple obese children and 134 healthy control children) in Liuzhou were enrolled.SNP rs1801282 of PPARγ2 gene were detected by SNaPshot assay.Height,weight and biochemical indicator of lipometabolism were measured.Metabolic parameters(FBG,TC,TG,HDL-C and VDL-C) and allele frequency were compared between the two groups. Results The CC and CG+GG genotype frequencies of rs1801282 polymorphism in normal control group and simple obesity group were 95.52%,4.48% and 98.01%,1.99%,respectively.The minor allele frequency(G allelic frequency) in simple obesity group and normal control group were 2.24%,and 0.99%,respectively.The genotype and allelic frequencies of rs1801282 and metabolic parameters(FBG,TC,TG,HDL-C and VDL-C) in simple obese children were not significantly different from those in controls (P>0.05).There were no significantly difference in male group and female group (P>0.05). Conclusions NP rs1801282 of PPARγ2 gene are not associated with simple obesity in Chinese Zhuang preschool children in Liuzhou of Guangxi province.
关键词
PPARγ /
-2基因 /
基因多态性 /
单纯性肥胖 /
壮族
Key words
PPARγ2 /
genetic polymorphism /
simple obesity /
Zhuang nationality
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基金
中国疾病预防控制中心妇幼保健中心合生元母婴营养与健康研究项目(2012FY009); 广西壮族自治区卫生厅自筹课题(Z2013602)
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