目的 探讨幼年期仔鼠母爱剥夺对成年期脑组织聚集DHA的影响。方法 C57BL/6J新生雄性仔鼠被随机分为母爱剥夺组和对照组,母爱剥夺组仔鼠在出生后第4~14天,每天与母鼠隔离6 h(9:00～15:00),对照组不接受任何处理。12周龄时,麻醉状态下心脏取血,采集脑组织。采用甲酯化法-气相色谱分析对血浆和脑中的脂肪酸谱进行检测;应用实时荧光定量PCR方法对肝脏和脑组织△5、△6去饱和酶的表达进行测定。结果 与对照组相比,母爱剥夺组仔鼠成年后血浆脂肪酸构成未发生明显变化,但肝脏和脑组织中二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)及总n-3 多不饱和脂肪酸(∑n-3 PUFAs)含量明显降低(P<0.05),二十二碳五烯酸(DPA)含量及∑n-6 PUFAs/∑n-3 PUFAs比值显著升高(P<0.05);同时,脑组织中△5去饱和酶与△6去饱和酶基因mRNA表达水平明显下降(P<0.05)。结论 生命早期遭受母爱剥夺的小鼠成年后脑聚集DHA减少,可能与脑组织中△5和△6去饱和酶基因表达抑制有关。
Abstract
Objective To explore the influences of maternal deprivation in young mice on their brain accretion of DHA in adult life. Methods Newborn C57BL/6J male pups were randomly divided into 2 groups:a maternal deprivation group (n=8) and a control group (n=8).The pups in the maternal deprivation group were separated from their mother for 6 hours (9:00~15:00) per day from postnatal day 4 to day 14,while the controls did not receive any intervention.The mice in both groups were sacrificed at the age of 12 weeks.The fatty acid composition in plasma and brain cortex was analyzed by the gas-chromatography.The expressions of delta 5 and delta 6 desaturases in the liver and brain were determined by the real-time PCR. Results Compared to the control group,the concentrations of DHA and n-3 PUFAs in brain were significantly decreased,whereas those of DPA were increased,leading to increased ratios of AA/DHA and n-6 /n-3 PUFAs in the deprivation group.However,no differences were shown in the plasma fatty acid composition between the maternal deprivation group and the control group.Comparing with the control group,the mRNA expression of cerebral delta 5 and delta 6 were significantly reduced in maternal deprivation group. Conclusion Maternal deprivation in early life reduces brain accretion of DHA in adult age,which may be resulted from the decreased desaturases' expressions.
关键词
母爱剥夺 /
n-3 多不饱和脂肪酸 /
二十二碳六烯酸 /
去饱和酶
Key words
maternal deprivation /
n-3 polyunsaturated fatty acids /
DHA /
desaturase
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基金
北京市科委行业定额经费自主项目(2015-bjsekyjs-Qi)
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