金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究

杨晓; 彭薇; 朱丽娜; 张小爱; 王艳

doi:10.11852/zgetbjzz2017-25-02-02

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中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (2) : 112-116. DOI: 10.11852/zgetbjzz2017-25-02-02

科研论著

金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究

杨晓¹,彭薇¹,朱丽娜¹,张小爱²,王艳¹

作者信息 +

A case-control study on the MMP-8C-799T and susceptibility to spontaneous preterm birth and premature rupture of membranes

YANG Xiao¹,PENG Wei¹,ZHU Li-na¹,ZHANG Xiao-ai²,WANG Yan¹

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文章历史 +

摘要

目的探讨金属蛋白酶8(MMP-8)基因C-799T多态性位点与自发性早产遗传易感性的关联性。方法病例组样本包括753例自发性早产(SPTB)新生儿,对照组包括681例足月新生儿。采用最新的Sequenom MassARRAY SNP检测技术对MMP-8基因C-799T多态性位点进行SNP分型。结果与携带MMP-8基因C-799T 多态性位点CC基因型的个体相比,携带至少一个-799 T等位型(CT+TT基因型)的个体发生SPTB、合并PROM的SPTB(PPROM)以及轻度早产的风险显著降低。与携带至少一个C等位型(CC+CT基因型)的个体相比,TT基因型与不合并PROM的SPTB的患病风险的降低有临界的相关性。结论 MMP-8基因C-799T多态性位点可以影响SPTB的遗传易感性。

Abstract

Objective To investigate the association between the genetic polymorphisms of MMP-8C-799T and susceptibility to spontaneous preterm birth (SPTB). Methods This case-control study enrolled 753 SPTB singleton neonates and 681 term neonates.Polymorphism was genotyped using Sequenom MassARRAY SNP. Result Compared with the CC genotypes,MMP-8-799T-positive genotypes (CT+TT genotypes) were significantly associated with a decreased susceptibility to SPTB and moderate SPTB.The -799T-positive genotypes (CT+TT genotype) were also significantly associated with decreased SPTB susceptibility in preterm neonates with PROM.Comparing with the CC + CT genotypes,-799TT genotype was marginally associated with a decreased SPTB susceptibility in preterm neonates without PROM. Conclusion MMP-8C-799T contributes to the SPTB susceptibility.

导出引用

杨晓,彭薇,朱丽娜,张小爱,王艳. 金属蛋白酶8基因 C-799T多态性位点与自发性早产遗传易感性的病例对照研究[J]. 中国儿童保健杂志. 2017, 25(2): 112-116 https://doi.org/10.11852/zgetbjzz2017-25-02-02

YANG Xiao,PENG Wei,ZHU Li-na,ZHANG Xiao-ai,WANG Yan. A case-control study on the MMP-8C-799T and susceptibility to spontaneous preterm birth and premature rupture of membranes[J]. Chinese Journal of Child Health Care. 2017, 25(2): 112-116 https://doi.org/10.11852/zgetbjzz2017-25-02-02

中图分类号： R179

参考文献

[1] Di RGC,Giardina I,Rosati A,et al.Maternal risk factors for preterm birth:a country-based population analysis[J].Eur J Obstet Gynecol Reprod Biol,2011,159(2):342-346.
[2] Hamilton BE,Martin JA,Ventura SJ.Births:preliminary data for 2012[J].Natl Vital Stat Rep,2013,62(3):1-20.
[3] Romero R,Velez EDR,Kusanovic JP,et al.Identification of fetal and maternal single nucleotide polymorphisms in candidate genes that predispose to spontaneous preterm labor with intact membranes[J].Am J Obstet Gynecol,2010,202(5):431.e1-34.
[4] Moura E,Mattar R,de Souza E,et al.Inflammatory cytokine gene polymorphisms and spontaneous preterm birth[J].J Reprod Immunol,2009,80(1-2):115-121.
[5] Velez DR,Fortunato SJ,Thorsen P,et al.Preterm birth in Caucasians is associated with coagulation and inflammation pathway gene variants[J].PLoS One,2008,3(9):e3283.
[6] Nagase H,Visse R,Murphy G.Structure and function of matrix metalloproteinases and TIMPs[J].Cardiovasc Res,2006,69(3):562-573.
[7] Weiss A,Goldman S,Shalev E.The matrix metalloproteinases (MMPS) in the decidua and fetal membranes[J].Front Biosci,2007,12:649-659.
[8] Nien JK,Yoon BH,Espinoza J,et al.A rapid MMP-8 bedside test for the detection of intra-amniotic inflammation identifies patients at risk for imminent preterm delivery[J].Am J Obstet Gynecol,2006,195(4):1025-1030.
[9] Wang Y,Yang X,Zheng Y,et al.The SEPS1 G-105A polymorphism is associated with risk of spontaneous preterm birth in a Chinese population[J].PLoS One,2013,8(6):e65657.
[10] Wang Y,Yang X,et al,A MCP-1 promoter polymorphism at G-2518A is associated with spontaneous preterm birth[J].Mol Genet Genomics,2015,290(1):289-296.
[11] 时春燕,漆洪波,杨慧霞.胎膜早破的诊断与处理指南(2015)[J].中华妇产科杂志,2015,3(1):3-8.
[12] Biggio JRJ,Ramsey PS,Cliver SP,et al.Midtrimester amniotic fluid matrix metalloproteinase-8 (MMP-8) levels above the 90th percentile are a marker for subsequent preterm premature rupture of membranes[J].Am J Obstet Gynecol,2005,192(1):109-113.
[13] Chaiworapongsa T,Hong JS,Hull WM,et al.Amniotic fluid concentration of surfactant proteins in intra-amniotic infection[J].J Matern Fetal Neonatal Med,2008,21(9):663-670.
[14] Park CW,Kim SM,Park JS,et al.Fetal,amniotic and maternal inflammatory responses in early stage of ascending intrauterine infection,inflammation restricted to chorio-decidua,in preterm gestation[J].J Matern Fetal Neonatal Med,2014,27(1):98-105.
[15] Shim SS,Romero R,Hong JS,et al.Clinical significance of intra-amniotic inflammation in patients with preterm premature rupture of membranes[J].Am J Obstet Gynecol,2004,191(4):1339-1345.
[16] Maymon E,Romero R,Chaiworapongsa T,et al.Amniotic fluid matrix metalloproteinase-8 in preterm labor with intact membranes[J].Am J Obstet Gynecol,2001,185(5):1149-1155.
[17] Wang H,Parry S,Macones G,et al.Functionally significant SNP MMP8 promoter haplotypes and preterm premature rupture of membranes (PPROM)[J].Hum Mol Genet,2004,13(21):2659-2669.
[18] Fortunato SJ,Menon R,Lombardi SJ.Amniochorion gelatinase-gelatinase inhibitor imbalance in vitro:a possible infectious pathway to rupture[J].Obstet Gynecol,2000,95(2):240-244.
[19] Beeghly-Fadiel A,Zheng W,Lu W,et al.Replication study for reported SNP associations with breast cancer survival[J].J Cancer Res Clin Oncol,2012,138(6):1019-2426.