目的 基于尿中对硝基酚 (para-nitrophenol,PNP)的生物监测评估儿童体内暴露负荷,为保护儿童健康提供科学依据。方法 选取江苏省某县参加“宫内环境化学物暴露对婴幼儿生长发育影响”队列的母亲于2009 年6 月-2010 年1月期间所产441名3岁幼儿作为研究对象,在其父母协助下完成问卷调查和尿样采集,采用气相色谱-串联质谱法(GC-MS/MS)检测儿童尿中PNP的含量,通过非参数检验和多元线性回归分析可能的影响因素,基于尿样监测数据评估并比较了儿童体内PNP负荷水平。结果 所有研究对象尿中均可检出对PNP,肌酐校正与未校正的PNP几何均值分别为12.14 μg/g cre.和5.08 μg/L。男童与女童PNP暴露水平未见统计学差异;多元线性回归结果显示,采样季节是影响PNP暴露水平的重要因素,夏季暴露水平高于其他季节,差异具有统计学意义(P<0.05),儿童尿中PNP浓度高于国外同类研究。结论 研究地区儿童普遍高暴露于PNP或其前体物质,其对儿童的潜在健康风险不容忽视。
Abstract
Objective To estimate exposure levels of urinary para nitrophenol (PNP) concentrations using biomonitoring data on 3-year-old children from an agricultural county,Jiangsu Province. Methods Questionnaires and spot urine samples were collected from 441 3-year-old children whose mothers participated our birth cohort study,which was named as "Effects of prenatal environmental chemicals exposure on the development of infants".The questionnaire survey included children's general demographic and socio-economic information and their lifestyles with potential exposure to PNP.Urinary PNP concentrations were measured using gas chromatography-tandem mass spectrometry (GC-MS/MS).Non-parametric test and multiple linear regression analysis were used to evaluate exposure of PNP. Results The PNP concentrations were detectable in all urinary samples.The geometric mean values of creatinine-adjusted and unadjusted were 12.14 μg/g cre.and 5.08 μg/L,respectively.Urinary concentrations of PNP in summer showed statistically higher than thoses of other seasons (P<0.05).No sex-specific difference was obtained in this study.PNP concentrations in children's urine were higher than other studies. Conclusions The findings suggested that the children are widely exposed to the PNP or precursor compounds at high levels in this county.The exactly biological mechanisms concerning the potential adverse effects on children deserve further investigations.
关键词
对硝基酚 /
暴露评估 /
生物监测 /
尿 /
儿童
Key words
para-nitrophenol /
exposure assessment /
biomonitoring /
urine /
children
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参考文献
[1] 张蕾,孙秋香.大孔树脂在高浓度有机废水处理中的应用[J].湖北第二师范学院学报,2008,27(8):51-54.
[2] Wan N,Gu J,Yan Y.Degradation of p-nitrophenol by Achromobacter xylosoxidans Ns isolated from wetland sediment[J].Int Biodeterior Biodegradation,2007,59(2):90-96.
[3] 周文敏,傅德黔,孙宗光.中国水中优先控制污染物黑名单的确定[J].环境科学研究,1991,4(6):9-12.
[4] Eichenbaum G,Johnson M,Kirkland D,et al.Assessment of the genotoxic and carcinogenic risks of p-nitrophenol when it is present as an impurity in a drug product[J].Regul Toxicol Pharmacol,2009,55(1):33-42.
[5] Li CM,Taneda S,Suzuki AK,et al.Estrogenic and anti-androgenic activities of 4-nitrophenol in diesel exhaust particles[J].Toxicol Appl Pharmacol,2006,217(1):1-6.
[6] Wang B,Yu G,Yu YJ,et al.Health risk assessment of organic pollutants in Jiangsu reach of the Huaihe River,China[J].Water Sci Technol,2009,59(5):907-916.
[7] Hryhorczuk D,Moomey M,Burton A,et al.Urinary p-nitrophenol as a biomarker of household exposure to methyl parathion[J].Environ Health Perspect,2002,110(Suppl 6):1041-1046.
[8] Qi X,Zheng M,Wu C,et al.Urinary pyrethroid metabolites among pregnant women in an agricultural area of the Province of Jiangsu,China[J].Int J Hyg Environ Health,2012,215(5):487-495.
[9] 姜帅,田雨来,刘强,等.江苏某县3岁儿童尿中重金属水平与生长发育的关系[J].环境与职业医学,2014,27(1):18-24.
[10] Lu D,Feng C,Wang D,et al.Analysis of twenty phenolic compounds in human urine:hydrochloric acid hydrolysis,solid-phase extraction based on K2CO3-treated silica,and gas chromatography tandem mass spectrometry[J].Anal Bioanal Chem,2015,407(14):4131-4141.
[11] Becker K,Seiwert M,Angerer J,et al.GerES IV Pilot Study:Assessment of the exposure of German children to organophosphorus and pyrethroid pesticides[J].Int J Hyg Environ Health,2006,209(3):221-233.
[12] Barr DB,Turner WE,Dipietro E,et al.Measurement of p-nitrophenol in the urine of residents whose homes were contaminated with methyl parathion[J].Environ Health Perspect,2002,110 (Suppl 6):1085-1091.
[13] Ruckart P,Kakolewski K,Bove FJ,et al.Long-term neurobehavioral health effects of methyl parathion exposure in children in Mississippi and Ohio[J].Environ Health Perspect,2004,112(1):46-51.
[14] Panuwet P,Prapamontol T,Chantara S,et al.Urinary pesticide metabolites in school students from northern Thailand[J].Int J Hyg Environ Health,2009,212(3):288-297.
[15] Arcury TA,Grzywacz JG,Isom S,et al.Seasonal variation in the measurement of urinary pesticide metabolites among Latino farmworkers in eastern North Carolina[J].Int J Occup Environ Health,2009,15(4):339-350.
[16] 赵敏娴,王灿楠,李亭亭,等.江苏居民有机磷农药膳食累积暴露急性风险评估[J].卫生研究,2013,30(5):844-848.
[17] 白红妍,韩彬,郑立,等.桑沟湾水体中有机磷农药残留组成与分布[J].岩矿测试,2012,31(4):632-637.
[18] 朱丽芳,周文军,王经委.杭嘉湖地区饮用水源有机农药污染现状调查与分析[J].浙江水利水电专科学校学报,2012,26(2):51-54.
[19] 于志勇,金芬,孙景芳,等.北京市场常见淡水食用鱼体内农药残留水平调查及健康风险评价[J].环境科学,2013,34(1):251-256.
[20] Roca M,Miralles-Marco A,Ferré J,et al.Biomonitoring exposure assessment to contemporary pesticides in a school children population of Spain[J].Environ Res,2014,131:77-85.
基金
国家“973”项目(2011CB503802)部分支持;上海市三年行动计划子课题职业健康风险监控和服务能力建设(GWIV-27.3)
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