目的 了解深圳地区汉族儿童维生素D受体(VDR)基因Bsm I和Fok I位点的多态性分布特点,为提高儿童科学补钙的依从性并促进儿童生长发育。方法 应用荧光定量PCR方法,检测233名深圳地区汉族儿童的VDR基因Bsm I和Fok I位点多态性,分析各基因型和等位基因频率分布,并与已报道的其他地区的数据进行比较。结果 本研究人群VDR Bsm I位点bb、Bb、BB基因型的分布频率分别为88.4%、11.6%、0,b、B等位基因频率分别为95.0%和5.0%;VDR Fok I位点ff、Ff、FF基因型的分布频率分别为18.6%、52.0%、29.4%,f、F等位基因频率分别为55.8%和44.2%。结论 深圳地区汉族儿童VDR Bsm I和VDR Fok I位点各基因型和等位基因的频率分布特征与已报道的上海、江苏地区差异有统计学意义。
Abstract
Objective To explore the polymorphism distribution of vitamin D receptor(VDR)gene Bsm I and Fok I in Han nationality children in Shenzhen,improve the compliance of children's scientific calcium and promote children's growth and development. Methods A total of 233 Han nationality children were recruited and collected oral epithelial cells.Genome DNA for fluorescence quantitative PCR to detect SNPs of Bsm I and Fok I were extracted.The Results were compared with the distribution frequencies of Bsm I and Fok I in other regions. Results The frequencies of Bsm I ( bb,Bb,BB ) in Han nationality children were 88.4%,11.6%,0 in Shenzhen,respectively.The frequency of Fok I ( ff,Ff,FF ) in Han nationality children were 18.6%,52.0%,29.4% in Shenzhen respectively. Conclusion Bsm I and Fok I polymorphism distribution in Han nationality children of Shenzhen city exhibit the own characteristics.
关键词
维生素D受体 /
基因多态性 /
汉族
Key words
vitamin D receptor /
gene polymorphism /
Han nationality
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基金
深圳市科技创新委员会项目基金(JCYJ20140416151506444)
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