目的 通过不间断喂养传代培养的Balb/c小鼠定量牛奶,观察牛奶对小鼠肠道乳糖酶表达量的影响。方法 传代培养Balb/c小鼠,实验组(S)给定量牛奶刺激,对照组(D)给予同等量的蒸馏水,分别在各代小鼠3,5,7,9周取小肠组织,石蜡包埋切片,选取兔源的抗乳糖酶I抗及荧光II抗进行免疫组织化学检测乳糖酶量的表达。每代小鼠随机选取40个样本,统计阳性率,以原代小鼠与实验组第4代对比,各周数小鼠横向对比,各代数纵向对比。结果 1)实验组各周数之间以第5周,第7周乳糖酶的表达量最高;2)第4代实验组小鼠的乳糖酶表达量较原代对照组小鼠表达量增加,结果有统计学意义(P=0.000 1);3)实验组各代数小鼠随代数增加,阳性率增加。结论 通过长期多代饮奶,实验组乳糖酶前体蛋白表达量较对照组增加,进一步验证乳糖耐受是可以被诱导的。
Abstract
Objective To observe the effect of milk on the mouse intestinal lactase expression quantity by continued to fed multi-generational Balb/c mice with milk. Methods Balb/c mice were cultured to the experimental group(S) given amount of milk passaged stimulation and control group (D) given the same amount of distilled water,respectively,in mice 3,5,7,9 weeks and small intestinal tissue was taken,as experimental material.Paraffin embedded sections,the source expression rabbit anti I and anti II anti lactase fluorescence immunohistochemical detection were selected to detect lactase amount.Each generation mice were randomly selected 40 samples,the positive rate of the primary mice and experimental group of fourth generation,horizontal comparison of each week number,and the algebra a longitudinal comparison. Results 1)The experimental group between the number of weeks,fifth weeks,seventh weeks had the highest expression of lactase.2)The fourth generation in the experimental group had the highest lactase expression level than the primary mice,there were statistical significance.3) The positive rate of the experimental group with algebra increased. Conclusion Through the long-term multi generation drinking milk,the experimental group lactase precursor protein expression is higher than that of control group,and further validate that lactose tolerance can be induced.
关键词
Balb/c小鼠 /
乳糖酶前体蛋白 /
乳糖不耐受 /
多代饮奶 /
动物传代
Key words
Balb/c mice /
lactase precursor protein /
lactose intolerance /
multi-generational drinking milk /
animal passage
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Carter SL,Attel S.The diagnosis and management of patients with lactose-intolerance[J].Nurse Pract,2013,38:23-28.
[2] Priebe MG,Wachters-Hagedoorn RE,Stellaard F,et al.Oro-cecal transittime:influence of asubsequent mea1[J].Eur J ClinInvest,2004,34:417-421.
[3] Enattah NS,Sahi T,Savilahti E,et al.Identification of a variant associated with adult-type hypo-lactasia[J].Nat Genet,2002,30:233-237.
[4] Tine GK,Jensen AL,Rikke L,et al.The -14010*C variant associated with lactase persistenceis located between an Oct-1 and HNF1 a binding site and increases lactase promoter activity[J].Hum Genet,2011,130:483-493.
[5] Ricardo A,Michael S,Torbjrn K,et al.Lactase non-persistence as a determinant of milk avoidance and calcium intake in children and adolescents[J].Journal of Nutritional Science,2013,2(26):1-5.
[6] Burger J,Kirchner M,Bramanti B,et al.Absence ofthelactase-persistence-associated allele in early Neolithic Europeans[J].Proc Natl Acad Sci USA,2007,104:3736-3741.
[7] Rejane M,Maria do SM,da Silva JMK,et al.A single singlenucleotide polymorphism is a better predictor of adult-type hypolactasia lactase persistence in Japanses-Brazilians than LCT-13910C/T[J].CLINICS,2010,65(12):1399-1400.
[8] XU LD,Sun HM,Zhang XL,et al.The -22018A allele matches the lactase persistence phenotype in northern Chinese populations[J].Scandinavian Journal of Gastroenterology,2010,45(2):168-174.
[9] Raja AHK,Mumtaz A,Babu R,et al.Thapa correlation of G/A -22018 single-nucleotide polymorphism with lactase activity and its usefulness in improving the diagnosis of adult-type hypolactasia among North Indian children[J].Genes Nutr,2013,8:145-151.
[10] Al-Abri A,Riad B.The phenotype/genotype correlation of lactase persistence among omani adults[J].Oman Medical Journal,2013,28(5):341-344.
[11] Byung JC,Jung HY,Yoo JC,et al.No association of LCT-13910 single nucleotide polymorphism with gastroenteritis in Korean children[J].Mol Cell Toxicol,2013,9:23-28.
基金
哈尔滨市科技创新人才研究专项资金项目(2012RFXXS027)
PDF(607 KB)
