目的 探讨骨髓基质细胞(bone marrow stromal cells,BMSCs)移植治疗对缺氧缺血性大鼠学习记忆能力的影响及相关机制。方法 取SD大鼠BMSCs进行培养,BrdU标记,经侧脑室植入7日龄缺氧缺血性大鼠模型中,8周后进行Morris水迷宫实验,水迷宫实验后取大鼠脑组织进行固定、脱水、包埋、切片;免疫组化行海马区神经元特异烯醇化酶标记及BrdU检测。结果 Morris水迷宫实验显示模型组大鼠T1和T2分别较假手术组大鼠缩短(P＜0.05);细胞组大鼠T1和T2分别较模型组大鼠延长(P＜0.05)。NSE免疫组化显示模型组大鼠海马阳性细胞积分光密度值(integral optical density,IOD)较假手术组IOD值减小(P＜0.05),细胞组IOD值较模型组明显增加(P＜0.05),细胞组BrdU免疫组化结果未见阳性细胞。结论 BMSCs经侧脑室植入可改善大鼠的学习记忆能力;其机制可能为注入侧脑室的BMSCs及相关物质促进自体神经元的再生。
Abstract
Objective To explore the therapeutic effect of transplantation of bone marrow stromal cells (BMSCs) on learning and memory ability of hypoxic ischemic rats and its related mechanism. Methods SD rats' BMSCs were cultured from femur in vitro,and labeled with BrdU,then implanted into 7 days old hypoxic-ischemic rats,the water maze experiment was tested after 8 weeks,then rats' brains were fixed,dehydrated,embedded,sliced,then did immunohistochemical staining and detected BrdU. Results Morris water maze showed T1 and T2 of the model group were shorter than those of the sham operation group (P＜0.05).T1 and T2 of cell group were longer than those of the model group (P<0.05).The integral optical density(IOD) value of immunoreative positive cells of NSE in model group was significantly lower than that of sham operation group (P＜0.05),IOD of cell group was higher than that of the model group (P＜0.05).But BrdU positive substance in cell group was bare. Conclusions Implanted BMSCs through lateral ventricle can improve the learning and memory ability of rats.The mechanism may be that BMSCs and related material injected into the lateral ventricle can promote neuron to regenerate.
关键词
骨髓基质细胞 /
移植 /
缺氧 /
缺血 /
大鼠 /
记忆
Key words
bone marrow stromal cells /
transplantation /
hypoxic /
ischemia /
rat /
memory
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参考文献
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基金
四川省泸州市科技局课题[2008-S-12(4/4)]
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