目的 观察矢车菊素-3-葡萄糖苷(cyanidin-3-glucoside, C3G)对肥胖大鼠血清炎症因子(TNF-α、IL-6和MCP-1)及胰岛素敏感性的影响。方法 3周龄雄性SD大鼠30只, 随机分为对照组(n=8)和高脂饮食组(n=22), 分别予以普通饲料及高脂饲料喂养5周。肥胖造模成功的17只大鼠再随机分为肥胖组(n=8)和C3G组(n=9)。C3G组予C3G 100 mg/(kg·d)灌胃, 其余组予等量生理盐水灌胃。实验结束时准确称量大鼠体重及内脏脂肪质量, 全自动生化分析仪测定空腹血糖(fasting glucose, FPG), 放免法测血清胰岛素(fasting insulin, FINS), ELISA测血清脂联素及TNF-α、IL-6、MCP-1水平, 并计算内脏脂肪比和胰岛素敏感指数(insulin sensitivity index, IAI)。结果 肥胖组和C3G组大鼠体重、内脏脂肪比和血清TNF-α、IL-6、MCP-1水平明显高于对照组(P均<0.05), 而C3G组上述指标明显低于肥胖组(P均<0.05)。肥胖组和C3G组血清脂联素水平及IAI明显低于对照组(P均<0.05), 而C3G组上述指标明显高于肥胖组上述指标。结论 C3G能明显增加肥胖大鼠的胰岛素敏感性, 其可能与增加血清脂联素水平及降低血清炎症因子(TNF-α、IL-6、MCP-1)水平有关。
Abstract
Objective To examine the effects of cyanidin-3-glucoside (C3G) on serum inflammatory cytokines and insulin sensitivity in diet-induced obese rats. Methods Thirty male Sprague-Dawley rats were randomly assigned to a control group (n=8) and a high fat diet group (n=22), and were fed with standard diet or high fat diet.Five weeks later, 17 high-fat diet-induced obese rats were assigned to a obese group (n=8) and a C3G group (n=9).Then, rats of obese group were treated with C3G at 100 mg/(kg·d) for 5 weeks.Body weight, visceral adiposity weight were measured, blood sample was collected for detecting levels of fasting glucose(FPG), fasting insulin(FINS), leptin, adiponectin, TNF-α, IL-6 and MCP-1, finally calculated visceral adiposity weight to body weight ratio(VBR) and insulin sensitivity index(IAI). Results Body weight, VBR, TNF-α, IL-6 and MCP-1 in the obese group were significantly higher than those of the control group (all P<0.05).The C3G group had significantly lower levels of body weight, VBR, TNF-α, IL-6 and MCP-1 than the obese group(all P<0.05), but the levels of the paremeters in the C3G group was significantly higher than that of the control group (P<0.05).Adiopnectin and insulin sensitivity index in the obese group was significantly lower than that of the control group (P<0.05), C3G treatment dramatically increased adiponectin levels and insulin sensitivity index compared with the obese group (P<0.05), although the levels of the two parameters did not reach to the levels of the control group. Conclusion Cyanidin-3-glucoside can increase insulin sensitivity through regulating serum inflammatory cytokines levels in obese rats.
关键词
矢车菊素-3-葡萄糖苷 /
低度炎症 /
胰岛素敏感性 /
脂联素 /
肥胖 /
大鼠
Key words
cyanidin-3-glucoside /
low-grade inflammation /
insulin sensitivity /
adiponectin /
obesity /
rats
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