目的 探讨支气管哮喘大鼠肺内c-fos蛋白表达和神经肽P物质(substance P, SP)和血管活性肠肽(vasoactive intestinal peptide, VIP)含量及分析之间的相关性。方法 32只SD大鼠随机分成正常对照组、哮喘非急性发作期组、哮喘急性发作组和地塞米松干预组。采用免疫组织化学进行组织学观察, 采用酶联免疫吸附试验法测定肺泡灌洗液中SP和VIP的浓度。结果 哮喘大鼠肺泡灌洗液中SP浓度较正常对照组显著增高(P＜0.000 1), VIP浓度显著降低(P＜0.000 1), 哮喘恢复组、哮喘急性期发作组及地塞米松干预组肺组织中c-fos蛋白光密度值与肺泡灌洗液SP浓度呈正相关(r分别为0.908、0.967、0.865, P<0.05或<0.001), 与肺泡灌洗液VIP浓度呈负相关(r分别为-0.974、-0.949、-0.962, P均≤0.001)。结论 哮喘大鼠肺内c-fos蛋白表达、神经肽含量与哮喘发作有关。
Abstract
Objective To investigate the change of distribution of c-fos and neuropetides in the lung of asthmatic rat. Methods Thirty-two Wistar rats were divided into control group, asthmatic recover group, acute episode group, dexameth intervention group.Immunohistochemistry and computer image analysis were used to observe the c-fos change of the lung and the contents of substance P(SP) and vasoactive intestinal peptide(VIP) in lung tissues were detected by ELISA. Results The contents of SP in the BALF of asthmatic recover group, acute episode group, dexameth intervention group rats were significantly higher than that in control group(P＜0.000 1), and the contents of VIP were significantly lower.There were significantly positive correlations between the SP and the optical density value of c-fos(r=0.908, 0.967, 0.865, P<0.05 or <0.001).There were significantly negative correlations between VIP and optical density value of c-fos (r=-0.974, -0.949, -0.962, P all ≤0.001). Conclusion There is correlation between neuropeptides, c-fos proteinum and asthmatic attack asthmatic rats.
关键词
支气管哮喘 /
c-fos蛋白 /
神经肽
Key words
bronchial asthma /
c-fos proteinum /
neuropeptide
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基金
陕西省科学技术研究发展计划项目[2007K-01(15)]
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