目的 探讨常压高氧对丙戊酸钠(valproic acid, VPA)孤独症模型鼠学习记忆能力及海马CA1区NF-κB阳性神经元表达水平的影响。方法 按Schneider方法制作VPA孤独症动物模型, 随机选取24只分为常压高氧模型组、模型对照组;随机选取同期Wistar孕鼠腹腔注射等量生理盐水后所产下的子代雄鼠作为正常对照组(每组12只)。采用Y型电迷宫测试大鼠学习记忆能力;采用免疫组化和图像分析技术检测模型鼠海马CA1区NF-κB阳性神经元表达水平。结果 常压高氧模型组治疗后大鼠学习记忆能力提高, 表现为Y型电迷宫检测尝试次数减少(31.08±0.99 vs 31.67±0.89, t=2.887, P=0.015), 再现次数增加(3.02±0.67 vs 2.62±0.52, t=-2.379, P=0.036);模型对照组与正常对照组比较, 海马CA1区NF-κB阳性细胞数增加(4.67±1.58 vs 3.00±1.54, t=2.639, P=0.015);常压高氧模型组与模型对照组比较, 海马CA1区NF-κB阳性细胞数减少(3.17±1.27 vs 4.67±1.58, t=2.588, P=0.017)。结论 孤独症的发病可能与海马CA1区NF-κB阳性神经元的表达水平有关, 常压高氧干预可降低模型鼠海马CA1区NF-κB阳性神经元的表达水平, 并改善模型鼠的学习记忆能力。
Abstract
Objective To observe the changes of learning and memory ability in valproic caid (VPA) autism model rats and the expression of NF-κB positive neurons in hippocampal CA1 region after normal pressure and high oxygen intervention. Methods To animal model of autism was establishe by the methods of Schneider, the subjects of study were randomly divided into normal pressure high oxygen group and model control group, normal control group were randomly selected from only males offspring of Wistar rat were evaluatepregnancy by intraperitoneal injection of the same amount of normal saline (each group 12).The learning and memory of the autism model rats were evaluated by the Y electricity maze test after the normal pressure and high oxygen intervention.The immunohistochemistry methods and image analysis were used to examine the number of NF-κB positive neurons in hippocampal CA1 region in different groups. Results The trying times of the normal pressure high oxygen group after treatment was less than those before treatment (31.08±0.99 vs 31.67±0.89, t=2.887, P=0.015), the memory times was increased(3.02±0.67 vs 2.62±0.52, t=-2.379, P=0.036).The number of the NF-κB positive neurons of the model control group was more than that of the normal control group, with statistical differences between them(4.67±1.58 vs 3.00±1.54, t=2.639, P=0.015).The number of the NF-κB positive neurons of the normal pressure high oxygen group was less than that of the model control group, with statistical differences between them(3.17±1.27 vs 4.67±1.58, t=2.588, P=0.017). Conclusions The pathogenesis of autism may be related to the expression level of NF-κB in the hippocampal CA1 region .The intervention of normal pressure high oxygen can reduce the expression of NF-κB in the hippocampal CA1 region of VPA autism model rats and improve their learning and memory ability
关键词
:孤独症 /
常压高氧 /
学习记忆 /
核转录因子κB /
大鼠
Key words
autism /
normal pressure high oxygen /
learning and memory /
nuclear transcription factor kappa B /
rats
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基金
青岛大学医学院附属医院2010年院内重点培育学科专项建设基金项目(2010-610)
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