宫内人巨细胞病毒感染对新生大鼠神经行为影响的追踪研究

潘娜娜; 王琍琍; 吴德; 唐久来

doi:10.11852/zgetbjzz2014-22-06-13

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中国儿童保健杂志 ›› 2014, Vol. 22 ›› Issue (6) : 600-604. DOI: 10.11852/zgetbjzz2014-22-06-13

基础科研论著

宫内人巨细胞病毒感染对新生大鼠神经行为影响的追踪研究

潘娜娜¹, 王琍琍¹, 吴德², 唐久来²

作者信息 +

Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.

PAN Na-na¹, WANG Li-li¹, WU De², TANG Jiu-lai².

Author information +

文章历史 +

摘要

目的探讨宫内人巨细胞病毒(human cytomegalovirus, HCMV)感染对新生大鼠神经系统发育的动态影响。方法建立宫内HCMV感染SD大鼠模型, 于1、3、6月龄时分别对实验组与对照组进行Morris 水迷宫、悬吊实验、斜坡实验、旷场实验等神经行为学检测, 实验前后分别采用光镜、电镜观察两组脑组织病理动态变化特点。结果与正常对照组比较, 实验组1、3、6月龄时的Morris 水迷宫逃避潜伏期、游泳距离均显著高于对照组, 同时其穿越平台次数显著低于对照组, 且随着年龄增加, 其穿越平台能力下降越明显(F=45.09, P<0.01);运动能力方面实验组大鼠1、3、6月龄, 悬吊实验得分(1.49±0.53、1.23±0.46、0.93±0.35)显著低于对照组(1.98±0.25、2.14±0.69、2.29±0.49), 差异均具有统计学意义(P均<0.001, 两独立样本非参数检验);表明宫内HCMV感染新生大鼠其运动能力显著降低;旷场试验结果实验组大鼠1、3、6月龄, 跨格子数(44.0±7.32、37.40±9.56、26.62±8.87)显著低于对照组(54.27±8.14、55.27±9.36、57.07±9.65), 差异具有统计学意义(P均<0.05)。病理结果显示随年龄增长, 实验组子代鼠的海马结构及神经髓鞘呈进行性破坏。结论宫内HCMV感染可以导致新生大鼠出现神经行为异常, 表现为学习记忆、运动功能和情绪行为发育的异常加重。

Abstract

Objective To detect the dynamic influence of nervous system development in neonatal rats by the intrauterine human cytomegalovirus (HCMV) infection. Methods The SD rat model of intrauterine HCMV infection was established, then the rats selected from experimental group and the control group randomly were done neuroethology test, such as Morris water maze test, hanging test, slope test, the open field test etc at the age of 1, 3 and 6 months respectively.Before and after the experiment, the light microscope and electron microscopy were used to observe their dynamic pathological change of brain tissue separately. Results Compared with normal control group, the Morris water maze escape latency, swimming distance of the experimental group at the age of 1, 3, 6 months were significantly higher than those of the control group, at the same time cross the platform number was significantly lower than control group, and with the increase of age, the cross platform ability was drop obviously (F=45.09, P<0.01);Motor functionrats' hanging test scores were (1.49±0.53), (1.23±0.46), (0.93±0.35) in infected group at the age of 1, 3 and 6 months, while those in the control group were (2.29±0.49), (2.14±0.69), (1.98±0.25), there were significant differences between two groups(samples nonparametric test, P all<0.001), suggested sport function of infected neonatal rat was decreased significantly.Open filed resultoffspring's across the grid number were (44.0±7.32), (37.40±9.56), (26.62±8.87) in infected group in 1, 3 and 6 months of age, while those in the control group were (57.07±9.65), (55.27±9.36), (54.27±8.14), there were significant differences between two groups (P all <0.05).Pathological results showed that along with the age growth, the hippocampus structure and nerve myelin of the experimental group offspring were aggravate gradually. Conclusion Intrauterine HCMV infection may result in the abnormal neural behavior of neonatal rat, and the impairment of learning and memory, motor function and emotional behavior development is aggravate gradually.

导出引用

潘娜娜, 王琍琍, 吴德, 唐久来. 宫内人巨细胞病毒感染对新生大鼠神经行为影响的追踪研究[J]. 中国儿童保健杂志. 2014, 22(6): 600-604 https://doi.org/10.11852/zgetbjzz2014-22-06-13

PAN Na-na, WANG Li-li, WU De, TANG Jiu-lai.. Tracing study on the neurobehavioral effects of neonatal rats by the intrauterine human cytomegalovirus infection.[J]. Chinese Journal of Child Health Care. 2014, 22(6): 600-604 https://doi.org/10.11852/zgetbjzz2014-22-06-13

中图分类号： R521.93

参考文献

[1] Buonsenso D, Serranti D, Gargiullo L, et al.Congenital cytomegalovirus infection:current strategies and future perspectives[J].Eur Rev Med Pharmacol Sci, 2012, 16(7):919-935.
[2] Swanson EC, Schleiss MR.Congenital cytomegalovirus infection:new prospects for prevention and therapy[J].Pediatr Clin North Am, 2013, 60(2):335-349.
[3] 李天宪, 赵林, 冯峰.人巨细胞病毒小鼠模型的建立[J].微生物学报, 1996, 36(4):292-294.
[4] Tang JL, Wang ML, Qiu JJ, et al.Building a mouse model hallmarking the congenital human cytomegalovirus infection in central nervous system[J].Arch Virol, 2002, 147(6):1189-1195.
[5] Lombardi G, Garofoli F, Mauro Stronati et al.Congenital cytomegalovirus infection:treatment, sequelae and follow-up[J].J Matern Fetal Neonatal Medi, 2010, 23(S3):45-48.
[6] 倪坤, 唐久来, 吴德, 等.孕早期人巨细胞病毒感染对仔鼠海马N-甲基-D-天冬氨酸受体亚基表达的影响[J].实用儿科临床杂志, 2012, 27(2):913-915.
[7] Jiang PF, Zhu T, Gao JD, et al.The effect of maternal infection on cognitive development and hippocampus neuronal apoptosis, proliferation and differentiation in the neonatal rats[J].Neuroscience, 2013, 246:422-434.
[8] Yoshihiro T, Hideyas, Kawasaki, et al.Reactivation of latent cytomegalovirus infection in mouse brain cell detected after Transfer to brain slice culture[J].Journal of Virology, 2002, 76(14):7247-7254.
[9] Townsend CL, Marianne FM, Ahlfors K, et al.Long-term outcomes of congenital cytomegalovirus infection in Sweden and the United Kingdom[J].Clin Infect Dis, 2013, 56(9):1232-1239.
[10] Bosnjak VM, Dakovi

I, Duranovi

V, et al.Malformations of Cortical Development in children with congenital cytomegalovirus infection -a study of nine children with proven congenital cytomegalovirus infection[J].Coll Antropol, 2011, 35(S):229-234.
[11] 孙竹风, 陈英.先天性巨细胞病毒感染导致脑损伤的临床分析[J].中国优生与遗传杂志, 2008, 16(5):123-125.
[12] Kawatani M, Nakai A, Okuno T, et al.Detection of cytomegalovirus in preserved umbilical cord from a boy with autistic disorder[J].Pediatr Int, 2010, 52(2):304-307.
[13] Yamashita Y, Fujimoto C, Nakajima E, et al.Possible association between congenital cytomegalovirus infection and autistic disorder[J].J Autism Dev Disord, 2003, 33(4):455-459.