Abstract: 【Objective】 To investigate the relationship between the polymorphisms for ACE gene and ApoE gene and the lipid metabolism,blood pressure in the chi1dren with simple obesity. 【Methods】 PCR-RFLP and polyacrylamide gelelectrophoresis were used to analyze the variation of the ACE gene and the ApoE gene of the 6~13 years old 105 simple obesity children(male 54 cases and female 51 cases)and the 98 healthy children(male 49 cases and female 49 cases).all children's TG,TC,HDLC,LDLC,blood pressure,height and weight were measured. 【Results】 The D-allele frequencies of ACE gene and the ε4 allele frequencies of ApoE gene in simple obesity children were higher(P<0.05).Whereas inheterozygote simp1e obesity children,the levels of the serum TG,TC,HDL-C and LDL-C,BMI,BP of the D/D and ε4 allele children were all different to the healthy children. 【Conclusions】 There are polymorphism of the ACE gene and the ApoE gene in children with obesity and health.The ACE gene and the ApoE gene have effects on lipid metabolisms,blood pressure in children with obesity and health children.

Key words: ACE gene, ApoE gene, obesity, lipid, blood pressure

摘要： 【目的】 探讨血管紧张素转换酶(angiotensin converting enzyme,ACE)基因和载脂蛋白E(apolipoprotein E,ApoE)基因多态性的分布及其多态性对健康和肥胖儿童的血压、脂质代谢影响。 【方法】 用PCR-RFLP及琼脂糖凝胶电泳法分析5~13岁的肥胖儿童(105例,男54人,女51人)及健康儿童(98例,男49人,女49人)ACE基因和ApoE基因的基因型,并测定两组儿童血清甘油三酯((TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)浓度、血压、身高和体重。 【结果】 肥胖儿童具有较高的D/D基因型频率、D等位基因频率及ε4等位基因频率。D/D型儿童与ε4等位基因携带儿童具有较高水平的血TG、TC、LDLC-C及收缩压(SBP)、舒张压(DBP)、BMI和较低水平的血HDL-C。 【结论】 本地区健康及肥胖儿童ACE和ApoE基因存在多态性,且ACE和ApoE基因多态性对健康儿童及肥胖儿童的血脂代谢、体脂分布及血压均有影响。

关键词: ACE基因, ApoE基因, 肥胖症, 血脂, 血压