Study on the changes of parameter of peripheral blood leucocytes in physical examination of children

doi:10.11852/zgetbjzz2017-25-09-18

Abstract

Abstract: Objective To analyze the morphological parameters of peripheral blood leucocytes in children,and to find the clinical significance.Methods A total of 180 healthy children (under 15 years old) were enrolled in this study,and parameters of white blood cells were analyzed by Sysmex XE-2100 automatic blood analyzer.The parameter data and changes of white blood cells were analyzed by age 0～,1～,2～,6～,12～15 years old.Results The fluorescence of neutrophilic granulocytes (NEUT-X) of all age groups were 1 355.0±41.5,1 348.3±22.5,1 348.4±51.3,1 339.3±51.6,1 335.8±46.2 respectively,and there were no statistical difference among groups (P=0.292).The fluorescence intensity of neutrophilic nucleic acid (NEUT-Y) were 495.6±28.2,477.6±38.6,486.5±40.2,481.3±32.2,479.9±39.6 respectively,and there were no statistical differences (P=0.412).The fluorescence intensity of lymphocyte nucleic acid (LYMPH-Y) were 713.6±46.3,708.3±60.3,711.0±61.2,712.8±72.3,711.6±75.3,and there were no significiant differences among groups (P=0.115).The fluorescence intensity of lymphocyte(LYMPH-X) were 896.3±36.2,854.6±19.3,858.4±17.6,860.1±15.7,851.3±18.2,and there were statistical differences among groups (P=0.008).Conclusion The granularity of peripheral blood lymphocyte in healthy children under 1 year old is higher than that of other ages,which indicated 0~1 years old children have better immunity.

Key words: leukocyte morphological parameters, peripheral blood of children, lymphocyte granularity, macrogranular lymphocytes

摘要： 目的分析儿童外周血白细胞形态参数的变化及其临床意义。方法设定运用Sysmex XE-2100全自动血细胞分析仪(简称XE-2100分析仪)检验血常规的180例门诊表观健康查体儿童(年龄≤15岁)为研究对象,回顾性收集其白细胞形态参数数据。按照年龄0～岁、1～岁、2～岁、6～岁、12～15岁分组,分析研究各白细胞形态参数的变化。结果研究观察各年龄组儿童中性粒细胞散射荧光度即颗粒度(NEUT-X)为1 355.0±41.5、1 348.3±22.5、1 348.4±51.3、1 339.3±51.6、1 335.8±46.2,各组之间差异无统计学意义(P=0.292);中性粒细胞核酸荧光强度即核的大小(NEUT-Y)为495.6±28.2、477.6±38.6、486.5±40.2、481.3±32.2、479.9±39.6,各组之间差异无统计学意义(P=0.412);淋巴细胞核酸荧光强度(LYMPH-Y)为713.6±46.3、708.3±60.3、711.0±61.2、712.8±72.3、711.6±75.3,各组间差异无统计学意义(P=0.115);淋巴细胞散射荧光强度(LYMPH-X)为896.3±36.2、854.6±19.3、858.4±17.6、860.1±15.7、851.3±18.2,各组间差异有统计学意义(P=0.008),其中0～岁组与其他各组两两比较差异有高度统计学意义(P＜0.001)。结论小于1岁的健康儿童外周血淋巴细胞颗粒度较其他年龄健康儿童高,具有较高的自身免疫抵抗能力。

关键词: 白细胞形态参数, 儿童外周血, 淋巴细胞颗粒度, 大颗粒淋巴细胞

CLC Number:

R179

ZHANG Lei,DUAN Xiao-jing,WANG Jin-hua,ZHANG Yan-ping,HUANG Lun,ZHANG Wen. Study on the changes of parameter of peripheral blood leucocytes in physical examination of children[J]. journal1, 2017, 25(9): 925-924.

张磊,段小静,王金华,张彦平,黄仑 ,张雯. 查体儿童外周血白细胞形态参数的变化及临床意义研究[J]. 中国儿童保健杂志, 2017, 25(9): 925-924.

References

[1] 武洪云.白细胞计数及分类的临床意义分析[J].中外健康文摘,2009,6(19):260-261.
[2] 杨春,蒋芙蓉,朱效茹,等.济南市区2～6岁健康儿童静脉血白细胞参数调查[J].中国医学创新,2016,13(27):53-55.
[3] 黄玉开,刘锐,顾晓琼,等.13442例儿童末梢血白细胞及五分类参数参考范围调查[J].国际检验医学杂志,2016,37(7):886-889.
[4] 孙宝苓,马丽娟,李静,等.健康儿童全血细胞分析11项参数年龄差异调查[J].中国妇幼健康研究,2012,23(3):268-270.
[5] 吴袁莉,王刚,陈葳.西安市2213例儿童静脉血细胞计数及其分类参考区间的建立[J].国际检验医学杂志,2016,37(8):1077-1078.
[6] Furundarena JR,Araiz M,Uranga M,et al.The utility of the Sysmex XE-2100 analyzer's NEUT-X and NEUT-Y parameters for detecting neutrophil dysplasia in myelodysplastic syndromes[J].International Journal of Laboratory Hematology,2010,32(3):360-366.
[7] Zhu Y,Cao X,Chen Y,et al.Neutrophil cell population data:useful indicators for postsurgical bacterial infection[J].Int J Lab Hematol,2012,34(3):295-299.
[8] Celikbilek A,Ismailogullari S,Zararsiz G.Neutrophil to lymphocyte ratio predicts poor prognosis in ischemic cerebrovascular disease[J].J Clin Lab Anal,2014,28(1):27-31.
[9] 顾青,邱丽君,周少春,等.血液细菌感染儿童白细胞体积、传导率、光散射参数变化的临床意义[J].诊断学理论与实践,2011,10(2):142-144.
[10] 崔颖,赵晓丹,申东晋,等.白细胞VCS参数在儿童支原体和细菌感染中的应用价值[J].中国实验诊断学,2014,18(1):53-55.
[11] 黄莉,夏曙华.感染性疾病患儿外周血大颗粒淋巴细胞观察[J].贵阳医学院学报,1999,24(1):62-63.
[12] 何国庆,张应权,刘云涛.小儿感染性疾病外周血大颗粒淋巴细胞临床分析[J].贵州医药,2002,26(3):253-253.
[13] 焦洋.中国健康成人不同年龄组外周血淋巴细胞亚群正常参考范围及其临床初步应用[D].北京:北京协和医学院,2009.

[1]	. [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1161-1162.
[2]	ZONG Xin'nan. History, achievements, problems and prospects of survey and research on physical growth and development of children in China [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1163-1168.
[3]	LI Dongdan, WU Jie. Individualized nutritional management strategies for food allergy in children [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1169-1172.
[4]	WANG Xi, LYU Huiling, HU Jiale, HAN Di, DING Ziyao, SHEN Hui, HU Jia. Association between the pubertal timing and high blood pressure in children and adolescents in Suzhou City [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1173-1178.
[5]	CAI Bingyao, CAO Ying, ZHU Siying, CAO Da, JIANG Jianuo, DONG Yanhui, MA Jun. Influence of lifestyle on overweight and obesity, abnormally high blood pressure and their morbidity among college students in northern China [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1179-1185.
[6]	DUAN Peifen, YUAN Jianhui, FENG Xiangxian. Effect of childhood obesity intervention on body shape perception and the mediating role of body shape perception in changes of obesity indicators [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1186-1189.
[7]	HUANG Kun, LI Xiuhong, WU Jiaqi, CHEN Jin, HAN Xiaopu, LIN Rong. Association between outdoor activity time of students and scoliosis [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1190-1195.
[8]	LIU Junting, LI Shaoli, CHEN Fangfang, ZONG Xinnan, HUANG Chunlei, ZHANG Tong, TAI Jun, GU Qinglong, On behalf of the Beijing Children and Adolescent Health Cohort Collaborative Group. Baseline characteristics analysis of the Beijing Children and Adolescents Natural Population Cohort [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1212-1218.
[9]	ZHANG Fen, JIA Lina. Research progress on food preferences of children and adolescents [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1219-1225.
[10]	WU Huahong, SHU Wen, YU Chengdong, ZHANG Yaqin, WU Ting, ZONG Xinnan. Meta-analysis of effect of calcium supplementation on children's height [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1246-1251.
[11]	WU Shuxian, WU Qingqing, WANG Lei, ZHAO Xiang, LYU Qiaohong, HUANG Yu. Pathway of the effect of screen exposure on overweight and obesity among children and adolescents [J]. Chinese Journal of Child Health Care, 2024, 32(11): 1252-1255.
[12]	DING Yonggang, LI Libo, MA Zhen, ZARXI Quzha. Non-suicidal self-injurious behaviors and associations with help-seeking behaviors among Tibetan middle school students in agricultural and pastoral areas of Tibet [J]. Chinese Journal of Child Health Care, 2024, 32(10): 1064-1069.
[13]	LIANG Guiye, GONG Jian, ZHOU Yue, GONG Linlin, WANG Yanni, XUE Hongli. Sleep health status and its influencing factors among primary and secondary school students in Lanzhou City [J]. Chinese Journal of Child Health Care, 2024, 32(10): 1070-1075.
[14]	JIN Guihua, CUI Wenxiang. Comparison of the detection rates of new media dependence and addiction among adolescents with different family social capital in the Yanbian area [J]. Chinese Journal of Child Health Care, 2024, 32(10): 1085-1090.
[15]	LI Tiangeng, GUO Liang, ZENG Sai, ZHENG Gangbin. Correlation of physical activity and sedentary behavior with cognitive development of preschool children [J]. Chinese Journal of Child Health Care, 2024, 32(10): 1111-1115.