Objective To evaluate the inhibition of the glutamate receptor blocker(GYKI52466) on the apoptosis of hypoxic ischemic encephalopathy(HIE) newborn rats' nerve cells. Methods In vivo:The newborn rats are randomly divided into blank control group (N),hypoxicischemic brain damage group (H) and GYKI52466 intervention group (G).Drug was given after modeling,the rats' neurological abnormality and ultrastructural change were observed of each group.In vitro:The newborn rat's brain cells were made into unicellular suspension liquid and cultivated for 4 days.Then group N cells were cultivated in normal environment,group H cells were cultivated in oxygen-glucose deprivation(OGD) environment,and group G cells were added with GYKI52466 after cultivated in OGD environment.After 6 hours,compared the cells growth state of each group,and analyzed the apoptosis of brain cells by flow cytometric. Results In vivo:The neurological behavior observation showed that group G cells' neurological abnormality was improved than group H cells;the electron microscopy observation showed,group H cells' neurons nuclei structure was damaged,nuclear membrane was ruptured,and nucleoli had mild shrinks.But group G cells' structure tended to be completed.In vitro:compared with model groups,the GYKI52466 treatment group's apoptotic cells were fewer,the numerical value had extremely significant difference (P<0.001). Conclusions Glutamate receptor blocker GYKI25466 can effectively reduce the abnormal neural behavior performance and pathology change for newborn rat with HIE,inhibit the apoptosis of HIE newborn rats' nerve cells.This test confirmed that GYKI25466 has neuroprotective effects on brain cells.
Key words
hypoxic-ischaemic encephalopathy /
glutamate /
AMPA /
GYKI52466
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