Abstract: Objective To explore the effect of perinatal high fat diet on uric acid metabolism in SD rat offspring. Methods Ten pregnant SD rats were randomly divided into control group and high fat diet group,fed with lab chow diet and high fat diet (containing 20% of lard) respectively.After weaning,half of male pups from either group were fed with lab chow and the other half were fed with high fat diet until 12 weeks of age.Then the body weight,level of serum uric acid,and activity of xanthine oxidase (XOD) and adenosine deaminase (ADA) were measured. Results The level of serum uric acid,the activity of XOD and body weight of the HF/HF group were higher than the B/B group and the HF/B group(P＜0.05).Compared with the B/B group,the level of serum uric acid and the activity of XOD were higher(P＜0.05).There was no difference of the activity of serum ADA among 4 groups. Conclusions Maternal high fat diet during gestation and lactation may cause hyperuricemia in rat SD offspring because of the increase of the activity of XOD.And the diet regulation on offspring after weaning can decrease the effect.

Key words: uric acid, high fat diet, xanthine oxidase, adenosine deaminase

摘要： 目的 探索孕哺期高脂膳食对子代SD大鼠血尿酸的影响。方法 10只成年雌性SD孕鼠随机分为对照组和高脂组,分别饲以标准鼠粮和含20%猪油的高脂饲料。4周龄断乳后,对照组雄性仔鼠中10只维持标准鼠粮(B/B),10只转饲以高脂饲料(B/HF);高脂组雄性仔鼠中10只维持原饲料(HF/HF),10只转饲以标准鼠粮(HF/B)。至12周龄,称量体重,测定血尿酸水平及黄嘌呤氧化酶和腺苷脱氨酶活性。结果 HF/HF组仔鼠体重、血尿酸水平及黄嘌呤氧化酶(xanthine oxidase,XOD)活性均显著高于B/B组及HF/B组(P＜0.05),HF/B组仔鼠血尿酸水平及XOD活性高于B/B组(P＜0.05),血ADA活性各组间差异无统计学意义(P>0.05)。结论 孕哺期高脂膳食可对子代高尿酸血症的发生产生影响,断乳后膳食调整可减弱其影响,机制可能与血黄嘌呤氧化酶活性的改变有关。

关键词: 高脂膳食, 尿酸, 黄嘌呤氧化酶, 腺苷脱氨酶