Abstract: 【Objective】 To discuss the relation between the protein expression of heme oxygenase-1(HO-1) and cytoglobin (CYGB),and whether hemin would reduce the brain damage against hypoxic-ischemic damage. 【Methods】 SD rats of 7-day old were divided into 5 groups randomly,including control group,sham-operated group,HIBD group,HIBD +Hemin group,and HIBD+ZnPP group.The rats of HIBD+Hemin group,HIBD group and HIBD+ZnPP group were given Hemin,normal saline or ZnPP 12 hours before the operation.The rats of three last groups were made for HIBD model.All the groups were killed at 0 h,24 h,48 h after HIBD.At 0 h,24 h,48 h after hypoxic-ischemic,observed the protein expression of HO-1 and CYGB in the brain. 【Results】 The protein expression of HO-1 and CYGB in all HIBD model groups were increased as time gone on.At any time after hypoxic-ischemic brain damage the expression of HO-1 and CYGB in the HIBD model groups were more than those in sham-operated group and control group.And in three HIBD model groups the expression of HO-1 and CYGB were showed by immunohistochemistry:HIBD+Hemin group>HIBD group>HIBD+ZnPP group,comparison of the three groups was with significant difference.The expression of HO-1 and CYGB had positive correlations in contex and hipppcampi in five groups.The relative factors r at 0h after HIBD were 0.687~0.916,24 h after HIBD were 0.473~0.903,48 h after were 0.659~0.962.The contex also had similar result. 【Conclusions】 Hemin which was the abduction of HO-1 could increase the expression of HO-1 in contex and hippocampus of HIBD+Hemin group.ZnPP which was the depressor of HO could decrease the expression of HO-1 of the HIBD+ZnPP group.The expression of HO-1 and CYGB was consistency,and they may have clear positive correlation.

Key words: cytoglobin, heme oxygenase-1, hypoxic-ischemic brain damage, relation

摘要： 【目的】 探讨血红素加氧酶(heme oxygenase,HO)与细胞红蛋白(cytoglobin,CYGB)表达的关系,为缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的防治提供实验依据。 【方法】 将7日龄新生SD大鼠170只随机分为正常对照组、假手术组、HIBD组、HIBD+Hemin组、HIBD+ZnPP组5组。其中HIBD组、HIBD+Hemin组及HIBD+ZnPP组在HIBD模型制备前12 h,分别腹腔注射生理盐水、Hemin及ZnPP,然后制备HIBD模型。术后于0、24、48 h采用免疫组化染色法分析HO -1及CYGB蛋白表达特点。 【结果】 缺氧后HO -1及CYGB表达随时间增长而递增,而且对于同一时间点HIBD+Hemin组HO -1及CYGB的表达均明显高于HIBD+ZnPP组,HIBD组居中,各组间比较差异有统计学意义;5组海马HO -1及CYGB表达均高度相关,0 h的r为0.687~0.916、24 h的r为0.473~0.903、48 h的r为0.659~0.962,皮质两者的表达亦有相同结果。 【结论】 Hemin诱导HO-1能使CYGB高表达,而ZnPP抑制HO-1则使CYGB表达减少;HO -1及CYGB的表达在HIBD时存在极明显的正相关性;实验证明脑缺氧缺血损伤时HO -1对CYGB的表达可能具有一定的正性调控作用。

关键词: 细胞红蛋白, 血红素加氧酶, 缺氧缺血性脑损伤, 表达关系