Expressions of serum soluble tumor necrosis factor related apoptosis inducing ligand and its receptor 1 and 4 in patients with bronchiolitis and acute upper respiratory infection

Abstract

Abstract: 【Objective】 To investigate the potential roles of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its soluble receptors (sTRAILR1 and sTRAILR4) in the pathogenesis of respiratory tract infections,the concentrations of sTRAIL,sTRAILR1 and sTRAILR4 proteins were determined. 【Methods】 Concentrations of sTRAIL and sTRAILR1 and sTRAILR4 protein were detected by ELISA,in patients with bronchiolitis and acute upper respiratory infection and individuals(named bronchiolitis group,AURI group,control group). 【Results】 The concentrations of sTRAIL,sTRAILR1 and sTRAILR4 protein were significantly higher in bronchiolitis group (85±38),(71±19),(167±97) pg/mL,respectively and in AURI group(78±28),(61±15),(139±72) pg/mL than those in control group(55±19),(45±14),(56±38) pg/mL,respectively(P all<0.01).Data also showed that the concentration of sTRAILR1 protein was markedly higher in bronchiolitis group than that in AURI group(P all<0.05).While no significance were observed of sTRAIL and sTRAILR4 expressions between the patients with bronchiolitis group and in AURI group(P>0.05).Furthermore,levels of sTRAILR1 was highly correlated to sTRAIL and sTRAILR4(n=102,r=0.236 and 0.409 respectively,all P<0.01). 【Conclusion】 Levels of sTRAIL,sTRAILR1 and sTRAILR4 were elevated in bronchiolitis and AURI which could be applied as an effective targets of patient's condition judgment.Our study indicated TRAIL and its receptors may involved in the pathogenesis of bronchiolitis and AURI inflammation.

Key words: enzyme linked immunosorbent assay, bronchiolitis, acute upper respiratory infection, tumor necrosis factor related apoptosis inducing ligand, receptor

摘要： 【目的】观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体(sTRAILR1和sTRAILR4)在毛细支气管炎和急性上呼吸道感染患儿中的表达,探讨它们在呼吸道感染炎症机制中的作用。【方法】 ELISA 法检测毛细支气管炎和急性上呼吸道感染患儿及健康对照组儿童血清sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度。【结果】毛细支气管炎组和急性上呼吸道感染组患者血清中sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度分别为(85±38)、(71±19)、(167±97);(78±28)、(61±15)、(139±72) pg/mL均显著高于对照组(55±19)、(45±14)、(56±38) pg/mL(P均<0.01);毛细支气管炎组sTRAILR1的蛋白浓度显著高于急性上呼吸道感染组(P<0.05),两组sTRAIL和sTRAILR4的蛋白浓度相比差异无统计学意义(P均>0.05)。sTRAILR1分别与sTRAIL、sTRAILR4的蛋白浓度呈显著正相关(n=102,r分别为0.236和0.409,P均<0.01)。【结论】毛细支气管炎和急性上呼吸道感染患儿血清中可溶性TRAIL及其受体TRAILR1和TRAILR4的表达增加,它们可能参与了呼吸道感染的炎症过程,TRAIL可能主要通过TRAILR1起作用,它们的表达水平增高可能被视为病情活动的指标之一。

关键词: 毛细支气管炎, 急性上呼吸道感染, 肿瘤坏死因子相关凋亡诱导配体, 受体, 酶联免疫吸附试验

CLC Number:

R725.6

JIANG De-fu,TAO Rong-hui,TONG Xia-sheng,CHEN Su-ping,WANG Ye-hua,WANG En-zhi. Expressions of serum soluble tumor necrosis factor related apoptosis inducing ligand and its receptor 1 and 4 in patients with bronchiolitis and acute upper respiratory infection[J]. journal1, 2012, 20(1): 75-77.

江德富,陶荣辉,童夏生,陈素萍,王晔华,王恩智. 毛细支气管炎和急性上呼吸道感染患儿血清可溶性TRAIL及其受体TRAILR1、TRAILR4的表达[J]. 中国儿童保健杂志, 2012, 20(1): 75-77.

References

[1] Cheong HJ,Lee KS,Woo IS,et al.Up-regulation of the DR5 Expression by Proteasome Inhibitor MG132 Augments TRAIL-Induced Apoptosis in Soft Tissue Sarcoma Cell Lines[J].Cancer Res Treat,2011,43(2):124-130.
[2] Guo L,Fan L,Ren J,et al.A novel combination of TRAIL and doxorubicin enhances antitumor effect based on passive tumor-targeting of liposomes[J].Nanotechnology,2011,22(26):265105-265107.
[3] Cziupka K,Busemann A,Partecke LI,et al.Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) improves the innate immune response and enhances survival in murine polymicrobial sepsis[J].Crit Care Med,2010,38(11):2169-2174.
[4] Stegmann KA,Bjrkstrm NK,Veber H,et al.Interferon-alpha-induced TRAIL on natural killer cells is associated with control of hepatitis C virus infection[J].Gastroenterology,2010,138(5):1885-1897.
[5] 胡亚美,江载芳.实用儿科学[M].7版.北京:人民卫生出版社, 2002:1169,1180.
[6] Thanaketpaisarn O,Waiwut P,Sakurai H,et al.Artesunate enhances TRAIL-induced apoptosis in human cervical carcinoma cells through inhibition of the NF-κB and PI3K/Akt signaling pathways[J].Int J Oncol,2011,39(1):279-285.
[7] 高志华,陈主初.TRAIL及其受体介导的信号转导通路[J].生命的化学,2001,21(3):212-214.
[8] Warke RV,Martin KJ,Giaya K,et al.TRAIL is a novel antiviral protein against dengue virus[J].J Virol,2008,82(1):555-564.
[9] Stegmann KA,Bjrkstrm NK,Veber H,et al.Interferon-alpha-induced TRAIL on natural killer cells is associated with control of hepatitis C virus infection[J].Gastroenterology,2010,138(5):1885-1897.
[10] Duttagupta PA,Boesteanu AC,Katsikis PD.Costimulation signals for memory CD8⁺ T cells during viral infections[J].Crit Rev Immunol,2009,29(6):469-486.
[11] Kotelkin A,Prikhod'ko EA,Cohen JI,et al.Respiratory syncytial virus infection sensitizes cells to apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand[J].J Virol,2003,77(17):9156-9172.
[12] Herold S,Steinmueller M,von Wulffen W,et al.Lung epithelial apoptosis in influenza virus pneumonia: the role of macrophage-expressed TNF-related apoptosis-inducing ligand[J].J Exp Med,2008,205(13):3065-3077.
[13] Brincks EL,Kucaba TA,Legge KL,et al.Influenza-induced expression of functional tumor necrosis factor-related apoptosis-inducing ligand on human peripheral blood mononuclear cells[J].Hum Immunol,2008,69(10):634-646.
[14] Robertson NM,Rosemiller M,Lindemeyer RG,et al.TRAIL in the airways[J].Vitam Horm,2004,67:149-167.

[1]	QIU Jiaqi , ZHONG Xia, ZOU Shipu. Role of dopamine and its receptors in tic disorder in children [J]. Chinese Journal of Child Health Care, 2024, 32(10): 1097-1100.
[2]	XIONG Ping, ZHU Hongmin, HUANG Xin, WU Jing, LIN Jing, ZHONG Yanyan. Association between genetic variation of ionized glutamate receptors and attention deficit hyperactivity disorder [J]. Chinese Journal of Child Health Care, 2023, 31(9): 945-950.
[3]	LI Jia, ZHANG Yanchi. Research advances on the correlation of gene polymorphisms of vitamin D receptor and vitamin D level with neurodevelopmental disorders in children [J]. Chinese Journal of Child Health Care, 2023, 31(5): 516-520.
[4]	WANG Xu, WANG Shu-ying, LI Li, ZOU Ying-xue. Meta-analysis of the risk factors for recurrent wheezing after bronchiolitis [J]. Chinese Journal of Child Health Care, 2023, 31(2): 190-194.
[5]	CUI Yifan, XIAO Xuwu. Association of vitamin D and its receptor gene polymorphisms with childhood diseases [J]. Chinese Journal of Child Health Care, 2023, 31(10): 1111-1115.
[6]	TIAN Bo, ZHAO Kai-hong, ZHAO Yi-ran, FU Hong-tao. Expression and its significance of peripheral blood mononuclear cells myeloid differentiation factor 88 and Toll-like receptor 4 in multiple organ dysfunction syndrome after asphyxia in premature infants [J]. Chinese Journal of Child Health Care, 2022, 30(4): 454-457.
[7]	TAO Feng-jiao, WEN Hang-wei, LIU Zuo-jiao. Relationship between serum high mobility group box-1 protein, soluble cluster differentiation 163 levels andfractional exhaled nitric oxide concentration and severity of bronchiolitis in children [J]. Chinese Journal of Child Health Care, 2022, 30(3): 335-338.
[8]	CHEN Guo-ping, ZHAO Ying-xin. Research advances in the role of Toll-like receptor 4 in neonatal necrotizing enteroeolitis [J]. Chinese Journal of Child Health Care, 2022, 30(2): 167-171.
[9]	ZHAO Ming, QIN Miao, SUN Meng-ya, JIANG Hong, LI Xiang-hong, JIANG Jian, LIU Yan. Study on the expression of NLRP3 and related inflammatory factors in necrotizing enterocolitis in newborn rats [J]. Chinese Journal of Child Health Care, 2021, 29(8): 852-856.
[10]	DONG Ming-ying, TAO Bing-tong. Correlation of leukotriene C4 synthase gene polymorphism with susceptibility and efficacy of respiratory syncytial virus bronchiolitis [J]. Chinese Journal of Child Health Care, 2021, 29(5): 545-549.
[11]	QIN Ying, YANG Shu-fen. Research progressin vitamin D and brain development [J]. Chinese Journal of Child Health Care, 2021, 29(4): 393-396.
[12]	WU Shao-hui, HUANG Xiao-dong, XU Zhi-hang. Analysis of the relationship between the expression of TLRs signaling pathway and inflammatory factors and recurrent respiratory tract infection [J]. journal1, 2021, 29(2): 205-208.
[13]	TANG Jing, LI Tao, MENG Lin. Predictive value of serum Toll-like receptor 4 for recurrence risk in children with severe asthma within one year after treatment [J]. Chinese Journal of Child Health Care, 2021, 29(12): 1372-1375.
[14]	ZHANG Juan-li, WANG Chun-yang, CHEN Min, AN Jian-feng, YAN Qin-qin. Effect and mechanism of down-regulation of Toll like receptor 4 mRNA on dendritic cell in hand-foot-mouth disease mice model [J]. journal1, 2020, 28(7): 748-751.
[15]	ZHANG Hui, LIU Chun-yan, WEI Dan. Mechanisms of S100A6 mediated by RAGE on vascular endothelial injury in obese children [J]. journal1, 2020, 28(2): 135-138.