Abstract: Objective To explore the levels of serum 25-(OH)D and vitamin B12 in children with autism spectrum disorders (ASD) and their correlation with disease characteristics,in order to provide a scientific basis for the prevention and treatment of ASD. Methods A total of 100 children with ASD treated in the First People's Hospital of Zhaoqing from June 2019 and June 2021 were retrospectively analyzed,and were selected as observation group.Another 100 healthy children who came to the hospital for physical examination were randomly enrolled as control group.The levels of serum 25-(OH)D and vitamin B12 of the children in two groups were compared,and the differences in supplementation rates of vitamin D and vitamin B12 during childhood and maternal pregnancy were statistically analyzed.Receiver operating characteristic (ROC) curve was used to analyze the differences in levels of serum 25-(OH)D and vitamin B12 of children in both groups.Spearman correlation analysis was adopted to explore the correlation between changes of levels of serum 25-(OH) D and vitamin B12 and severity of ASD. Results The levels of serum 25-(OH)D and vitamin B12 of children,the supplementation rates of vitamin D and vitamin B12 of children and mothers in the observation group were significantly lower than those in control group (t=9.230,7.163, χ²=16.647,23.894,11.524,4.760,P<0.05).The levels of serum 25-(OH)D and vitamin B12 had the value of predicting and evaluating ASD (AUC=0.816,0.761,P<0.05).The levels of serum 25-(OH)D and vitamin B12 of children with mild-to-moderate ASD were higher than those with severe ASD (P<0.05).Spearman correlation analysis found that the severity of ASD children was negatively correlated with levels of serum 25-(OH)D and vitamin B12 (r_s=-0.328,-0.301,P<0.05). Conclusions Children with ASD have abnormal serum 25-(OH) D and vitamin B12.The supplementation rates of vitamin D and vitamin B12 during childhood and maternal pregnancy are related to the incidence of ASD.Vitamin D and B12 supplementation during pregnancy may have a certain effect on preventing the treating of ASD in children.

Key words: autism spectrum disorders, 25-(OH)D, vitamin B12

摘要： 目的 探究孤独症谱系障碍(ASD)儿童血清25-(OH)D和维生素B12水平及其与病情特征的相关性,为ASD的预防及治疗提供科学依据。方法 回顾性分析2019年6月-2021年6月于肇庆市第一人民医院收治的ASD儿童100例作为观察组,随机选取在本院体检的健康儿童100例为对照组。比较两组患儿血清25-(OH)D、维生素B12水平,统计两组患儿及母亲孕期维生素D及维生素B12补充率水平差异,使用特征(ROC)曲线分析两组患儿血清25-(OH)D、维生素B12水平差异。使用Spearman相关性分析血清25-(OH)D、维生素B12水平变化与ASD严重程度的相关性。 结果 观察组患儿血清25-(OH)D、维生素B12水平、患儿及母亲孕期维生素D及维生素B12补充率均低于对照组(t=9.230、7.163,χ²=16.647、23.894、11.524、4.760,P＜0.05)。血清25-(OH)D、维生素B12水平均具备预测、评估ASD的价值(AUC=0.816、0.761,P＜0.05)。轻中度组患儿血清25-(OH)D、维生素B12水平均高于重度组(P＜0.05)。经Spearman相关性分析,ASD患儿病情严重程度与血清25-(OH)D、维生素B12水平负相关(r_s=-0.328、-0.301,P＜0.05)。 结论 ASD儿童存在血清25-(OH)D、维生素B12异常,患儿及母亲孕期维生素D及B12补充率水平与ASD发病存在一定联系。ASD患儿及母亲孕期补充维生素D与B12可能对ASD的防治有一定效果。

关键词: 孤独症谱系障碍, 25-(OH)D, 维生素B12