Objective To observe the effect of milk on the mouse intestinal lactase expression quantity by continued to fed multi-generational Balb/c mice with milk. Methods Balb/c mice were cultured to the experimental group(S) given amount of milk passaged stimulation and control group (D) given the same amount of distilled water,respectively,in mice 3,5,7,9 weeks and small intestinal tissue was taken,as experimental material.Paraffin embedded sections,the source expression rabbit anti I and anti II anti lactase fluorescence immunohistochemical detection were selected to detect lactase amount.Each generation mice were randomly selected 40 samples,the positive rate of the primary mice and experimental group of fourth generation,horizontal comparison of each week number,and the algebra a longitudinal comparison. Results 1)The experimental group between the number of weeks,fifth weeks,seventh weeks had the highest expression of lactase.2)The fourth generation in the experimental group had the highest lactase expression level than the primary mice,there were statistical significance.3) The positive rate of the experimental group with algebra increased. Conclusion Through the long-term multi generation drinking milk,the experimental group lactase precursor protein expression is higher than that of control group,and further validate that lactose tolerance can be induced.
Key words
Balb/c mice /
lactase precursor protein /
lactose intolerance /
multi-generational drinking milk /
animal passage
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