关键词: 母爱剥夺, n-3 多不饱和脂肪酸, 二十二碳六烯酸, 去饱和酶

Abstract: Objective To explore the influences of maternal deprivation in young mice on their brain accretion of DHA in adult life. Methods Newborn C57BL/6J male pups were randomly divided into 2 groups:a maternal deprivation group (n=8) and a control group (n=8).The pups in the maternal deprivation group were separated from their mother for 6 hours (9:00~15:00) per day from postnatal day 4 to day 14,while the controls did not receive any intervention.The mice in both groups were sacrificed at the age of 12 weeks.The fatty acid composition in plasma and brain cortex was analyzed by the gas-chromatography.The expressions of delta 5 and delta 6 desaturases in the liver and brain were determined by the real-time PCR. Results Compared to the control group,the concentrations of DHA and n-3 PUFAs in brain were significantly decreased,whereas those of DPA were increased,leading to increased ratios of AA/DHA and n-6 /n-3 PUFAs in the deprivation group.However,no differences were shown in the plasma fatty acid composition between the maternal deprivation group and the control group.Comparing with the control group,the mRNA expression of cerebral delta 5 and delta 6 were significantly reduced in maternal deprivation group. Conclusion Maternal deprivation in early life reduces brain accretion of DHA in adult age,which may be resulted from the decreased desaturases' expressions.

Key words: maternal deprivation, n-3 polyunsaturated fatty acids, DHA, desaturase