Objective To explore the clinical characteristics of neonatal stroke (NS) and provide some theoretical references for its diagnosis and treatment. Methods The clinical data of 37 cases of NS retrospectively were analyzed, including various perinatal factors, clinical manifestations and neuroimaging findings. Results 1)In 37 cases, 31 cases (83.8%) presented with significant nervous system symptoms and signs, including 24 patients (77.4%) started with convulsion or being as the main clinical manifestation, 15 patients (62.5%) had the significance of fixed position.10 cases (32.3%) were with weakening primary reflexes.But 6 patients (16.2%) did not present with any obvious neurological abnormal manifestations, cerebral infarction changes could be found only by head imaging examinations.2)The earliest onset time was 10 minutes after birth, while the latest was 12 days after birth.23 cases of the 31 patinets(74.2%) were attacked by NS within 24 hours after birth, 27 cases(87.1%)within 48 hours and 28 cases(90.3%)within 72 hours.3)26 patients(70.3%) were given by cesarean delivery, 11 patients(29.7%) were given by natural childbirth.4)16 patients(43.2%)were with the history of perinatal hypoxia and 10 patients(27.0%) were with premature rupture of membranes (PROM) or intrauterine infection.5) 18 patients (48.6%) were injured in bilateral hemisphere, 12 cases (32.4%) in left hemisphere, 7 cases (18.9%) in right hemisphere and 7 cases (18.49) in the basal ganglia. Conclusions 1)Convulsion is the most common clinical manifestation of NS.2)Early onset.3)Cesarean section is closely correlated with NS, accounting for more than 70%.4)Perinatal hypoxia , PROM and intrauterine infection are the most common reasons of NS.5)Bilateral hemisphere simultaneously involved infarction is the commonest, accounting for nearly 50%.In unilateral hemispheric infarction, the left side suffered more than the right side, basal ganglia infarction simultaneously existed in nearly 20% of NS patients.6)More than 60% of the patients were with poor prognosis.7)The diagnosis of NS depends on the imaging examinations, head B ultrasound is a prior choice, but further diagnosis depends on MRI-DWI.