Analysis of gene mutation  in surfactant protein B +1580 site on full-term newborns with respiratory distress syndrome.

YIN Xiao-juan; HAN Xiao; FAN Han-xiao; XIE Lu; QU Wen-wen; FENG Zhi-chun.

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Chinese Journal of Child Health Care ›› 2013, Vol. 21 ›› Issue (7) : 682-684.

Analysis of gene mutation in surfactant protein B +1580 site on full-term newborns with respiratory distress syndrome.

YIN Xiao-juan,HAN Xiao,FAN Han-xiao,XIE Lu,QU Wen-wen,FENG Zhi-chun.

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Abstract

Objective To analyze correlative factor between gene mutation (SP-B +1580) and respiratory distress syndrome(RDS) in full-term newborns. Methods There were 312 Han full-term newborns with RDS as RDS group and 156 Han healthy full-term newborns as control group.Genotypic analysis on exon 4 was performed with polymerase chain reaction amplification(PCR) and gene sequencing.Analysis of for expression of SP-B and proSP-B in lung were determined with immunohistochemical technique. Results Three genotypes of SP-B+1580 including CC,C/T,TT were discovered in two groups.Frequency of C/T genotype in RDS group was higher than that in control group(P<0.05).Patients with C/T genotype had higher rates on uterine-incision delivery mechanical ventilation and mortality chest X-ray grade showed severity about RDS,and needed more pulmonary surfactants(P<0.01).19 patients with decreased mature SP-B and proSP-B were among 50 cases of dead patients with C/T genotypes. Conclusion C/T genotype in SP-B +1580 site causing decreased mature SP-B and proSP-B may be the high risk for RDS.

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respiratory distress syndrome / full-term / newborn / surfactant protein B / gene mutation

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YIN Xiao-juan,HAN Xiao,FAN Han-xiao,XIE Lu,QU Wen-wen,FENG Zhi-chun.. Analysis of gene mutation in surfactant protein B +1580 site on full-term newborns with respiratory distress syndrome.[J]. Chinese Journal of Child Health Care. 2013, 21(7): 682-684

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