Objective To research bifidobacterium's effects on tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in intestinal tissue of neonatal SD rat model of necrotizing enterocolitis (NEC) and to investigate the protective effects of bifidobacterium against NEC. Methods 128 neonate Sprague-Dewley (SD) rats aged 2 days were divided into four groups at random:LPS group(A),LPS+bifidobacterium group(B),normal saline group (C) and bifidobacterium group(D).After received injection of LPS for a week,eight rats,which were selected form each group at random,were killed quickly respectively at 2,6,12 and 24 hours through decollation.The intestinal tissue obtained,the ileocecal junction was for histological analysis,and the remains was used to measure the level of TNF-α and IL-10 through ELISA method. Results Compared to group A,the scores of intestinal histologic pathological injury of group B at 2,6,12,24 hours were significantly lower (P<0.05) and the levels of TNF-α in ileal tissue of group B at 2,6,12 hours were significantly lower(P<0.05).Also compared to group A,the levels of IL-10 in ileal tissue of group B at 2,6,12,24 hours were significantly higher (P<0.05).Statistics showed that TNF-α level had positive correlation with the scores of neonatal rats of intestinal histologic injury (r=0.646) and IL-10 level had negative correlation with the scores of neonatal rats of intestinal histologic injury (r=-0.598). Conclusion Bifidobacterium could reduce the level of TNF- α and increase the level of IL-10 of NEC model,bifidobacterium provides protection from NEC.
Key words
bifidobacterium /
necrotizing enterocolitis /
LPS /
TNF-α /
IL-10
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