Objective To observate change of amplitude integrated electroencephalography(aEEG) on neonatal hypoxic-ischemic encephalopathy(HIE),the relationship were respectively studied between the aEEG classification and HIE clinical index,HIE head CT dividing,to explore the diagnostic value of aEEG in HIE. Methods A total of 48 HIE newborns who had asphyxia were as the research objects.According to the clinical dividing into mild HIE group of 20 cases,moderate HIE group of 16 cases,and severe HIE group of 12 cases.According to the head CT dividing into mild HIE group of 18 cases,moderate HIE group of 18 cases,and severe HIE group of 12 cases.According to the aEEG classification standard the research objects were divided into normal,mildly abnormal and severe abnormal.aEEG characteristics were observed on 48 cases HIE newborns. Results aEEG abnormal rate of newborns with mild clinical HIE was 13.3%,aEEG abnormal rate of newborns with moderate clinical HIE was 86.7%,and aEEG abnormal rate of newborns with severe clinical HIE was 100.0%.Abnormal degree of aEEG and clinical index of HIE was closely related (r=0.853,P<0.000 5);aEEG abnormal rate of newborns with mild HIE in head CT was 10.5%,aEEG abnormal rate of newborns with moderate HIE in head CT was 82.4%,aEEG abnormal rate of newborns with severe HIE in head CT was 100.0%.Abnormal degree of aEEG and head CT dividing of HIE was closely related (r=0.825,P<0.000 5). Conclusion Abnormal degree of aEEG and clinical index in HIE,head CT dividing in HIE have close positive correlation,and aEEG has great application value in early diagnosis of HIE,judgment condition of HIE and prognosis prediction of HIE.