Effect of pre-gestational stress on learning and memory function and the expression of synaptotagmin I and synaptotagmin IV in hippocampus of the offspring rats

HUANG Yue-jun; SHI Xue-chuan; CHEN Si-hong; YANG Han-hua; LAI Hui-hong; LI Bi-yan.

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Chinese Journal of Child Health Care ›› 2013, Vol. 21 ›› Issue (1) : 46-49.

Effect of pre-gestational stress on learning and memory function and the expression of synaptotagmin I and synaptotagmin IV in hippocampus of the offspring rats

HUANG Yue-jun,SHI Xue-chuan,CHEN Si-hong,YANG Han-hua,LAI Hui-hong,LI Bi-yan.

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Abstract

Objective To investigate the effect of pre-gestational stress on the spacial memory of the offspring rats as well as the expression of synaptotagmin I (syt I) and synaptotagmin IV (syt IV) in hippocampus when they were one month old. Methods Adult female SD rats were divided randomly into two groups:control group (n=8) and chronic unpredictable stress (CUS) group (n=12).The spacial memory of the offspring rats was tested in Morris water maze when they were one month old.Serum cortisol (COR) and corticotropin-releasing hormone (CRH) levels of maternal rats and offspring rats were determined by using a standard Elisa kit.The expression of syt I and syt IV in the hippocampus of the offspring rats were studied by immunohistochemistry. Results 1)After the stress procedure and post-delivery,maternal serum CRH and COR level in CUS group were higher than control group.2)Morris water maze test showed that the escape latency of offspring rats in CUS group were longer than in control group; Number of times that the rat crossed the position of offspring rats in CUS group were less than in control group.3)The CRH and COR level of offspring rats in CUS group were higher than in control group.4)There were statistical differences of the mean optical density (MOD) of syt I and syt IV at GA3,DG in the offspring rats between cus group and control group (All P<0.05). Conclusion Pre-gestational stress impaires the spacial memory of the offspring rats and reduces the expressions of syt I and syt IV in hippocampus of them.

Key words

chronic unpredictable stress / learning / cortisol / corticotropin-releasing hormone / synaptotagmin I / synaptotagmin IV

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HUANG Yue-jun,SHI Xue-chuan,CHEN Si-hong,YANG Han-hua,LAI Hui-hong,LI Bi-yan.. Effect of pre-gestational stress on learning and memory function and the expression of synaptotagmin I and synaptotagmin IV in hippocampus of the offspring rats[J]. Chinese Journal of Child Health Care. 2013, 21(1): 46-49

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