Clinical significance of serum tumor necrosis factor and it's soluble receptor 1 and 2 in patients with bronchial asthma
- TONG Xia-sheng1, LI Chen-hong1, FANG Li2, WANG En-zhi1, YE Hui3
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Abstract
【Objective】 To investigate the potential roles of tumor necrosis factor-α(TNF-α) and it's solube receptors(sTNF-R1 and sTNF-R2) in the pathogenesis of asthmatic inflammation, the concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were determined. 【Methods】 All the patients were obtained including 46 cases of patients with bronchial asthma exacerbation, 31 cases of patients with bronchial asthma paracmasia, and were compared to 22 cases of individuals. Concentrations of TNF-α proteins was detected by enzyme linked immunosorbent assay methods(ELISA). And the concentrations of sTNF-R1 and sTNF-R2 proteins were also determined by ELISA. 【Results】 The concentrations of TNF-α, sTNF-R1 and sTNF-R2 proteins were significantly higher in asthma exacerbation group[(3.19±1.68),(4.69±2.30),(14.32±6.19) ng/mL respectively] than those in control group [(1.96±0.86)、(2.07±0.86)、(4.75±1.68) ng/mL respectively](all P<0.01). Data also showed that the concentrations of sTNF-R1 and sTNF-R2 proteins were markedly lower in bronchial asthma paracmasia group [(2.59±1.21)、(11.57±4.78) ng/mL respectively] than those in asthma exacerbation group(P＜0.01 or ＜0.05, respectively). While no significance were observed of TNF-αexpressions between the patients with asthma paracmasia group(2.75±1.31) ng/mL and in acute phase(P>0.05). When compared to control group, the concentrations of TNF-α and sTNF-R2 proteins in asthma paracmasia group were significantly higher, but there were no significance of sTNF-R1 proteins between them. Furthermore, levels of sTNF-R1 was highly correlated to sTNF-R2(n=99,r=0.239,P＜0.05), but there were no strong association neither between levels of sTNF-R1 and TNF-α, nor between levels of sTNF-R2 and TNF-α(all P>0.05). 【Conclusions】 Levels of TNF-α, sTNF-R1 and sTNF-R2 are elevated in bronchial asthma which could be applied as an effective targets of patient's condition judgment. Our study indicated TNF-α, sTNF-R1 and sTNF-R2 expressions may involved in the pathogenesis of bronchial asthma inflammation.
Key words
bronchial asthma / tumor necrosis factor / receptor / enzyme linked immunosorbent assay
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References
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[9] Sikora JP, Kuzański W, Andrzejewska E. Soluble cytokine receptors sTNFR I and sTNFR II, receptor antagonist IL-1ra, and anti-inflammatory cytokines IL-10 and IL-13 in the pathogenesis of systemic inflammatory response syndrome in the course of burns in children[J]. Med Sci Monit,2009,15(1):26-31.
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[12] Sumitomo M, Suda S, Shindo K, et al. Serum levels of tumor necrosis factor alpha and soluble tumor necrosis factor-receptor I in asthmatic patients and patients with chronic respiratory tract infection[J]. Arerugi,1997,46(11):1136-1147.
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