Objective To understand the physical fitness level among the preschoolers in Shuangliu district,and to explore the gender difference and age difference as well as development characteristics. Methods Stratified cluster sampling was adopted to select participants,and physical fitness examinations were conducted according to national physical fitness examination standard manual. T test was performed to compare the physical fitness aggregate score between different gender groups. Variance analysis was used to compare the physical fitness aggregate score between different age groups and Chi-square test was used to analyze the distribution of overall ranking between different ages and genders. Results A total of 1 077 preschoolers aged 3~6 were included in the study. Qualification percentage of physical fitness examination in the participants was 87.16%. As concerned with aggregate score,t test showed that statistical difference was detected in physical fitness aggregate score between different ages in both gender groups .Total score of physical fitness were different in 3.5-year-old group,4-year-old group,and 4.5-year-old group between boys and girls(Girls′physical fitness aggregate scores were higher than those of boys). As concerned with integrated ranking,Chi-square test showed that statistical difference was existed in integrated ranking distribution between different ages in both gender groups; Age groups of highest excellence rates were 5.5-year-old group and 4.5-year-old group. Girls′excellence rates were higher than those of boys in all age groups while only the difference of 4.5-year-old group was statistically significant. Conclusions Physical fitness level among preschoolers in Shuangliu district is at a lower level; Intervention among preschoolers should be tailored and individualized according to corresponding age,gender and indicators in order to enhance physical fitness level among preschoolers.